摘要
目的:观察中药水煎剂在对抗模拟失重大鼠血循环紊乱、骨质疏松及肌肉萎缩的作用。方法:实验于2000-05/2002-05在航天医学工程研究所第5研究室失重生理学实验室完成。健康SD雄性大鼠120只,随机数字法分为6组:正常对照组;单纯尾吊组(采用大鼠头低位30°尾部悬吊模型模拟失重对机体的影响);丹黄刺组(尾吊+丹参、黄芪、刺五加水煎剂);参川熟组(尾吊+人参地上总甙、川芎、熟地水煎剂);参山杜组(尾吊+山楂、西洋参、杜仲水煎剂);参银生组(尾吊+人参根、银杏、生地、水煎剂)。实验第30天,经心脏取血10mL,取肌肉及骨骼,测定①血循环指标:全血黏度、红细胞变形性、纤维蛋白原含量、血细胞压积、红细胞形态。②肌肉质量,Ⅰ和Ⅱ型肌纤维比例和面积。③骨骼生物力学特性及矿盐含量。结果:①单纯尾吊导致大鼠肌肉质量、肌肉纤维面积、Ⅰ型纤维比例显著下降(P<0.05～0.001);骨皮质面积、矿盐含量、生物力学参数等也明显降低(P<0.05);大鼠血液流变学参数发生明显改变——中切与低切血黏度、血浆纤维蛋白原含量、异形红细胞比例增加[中切血黏度:(8.25±1.70),(6.44±0.88)mPa·s;低切血黏度:(25.10±8.93),(10.87±5.71)mPa·s,P<0.05或0.001];红细胞最大变形指数、积分变形指数及血细胞压积下降[最大变形指数:(60.69±1.20)%,(64.73±2.66)%;积分变形指数:(42.31±2.97)%,(45.00±1.80)%,P<0.01～0.001]。②中药复方组与单纯尾吊组相比,血液流变学指标均有较大改善,并可显著增加尾吊大鼠腓肠肌质量,对部分骨生物力学参数也有作用[中切血黏度:丹黄刺组(4.41±1.01)mPa·s,参川熟组(6.32±1.14)mPa·s,参山杜组(6.68±0.52)mPa·s;低切血黏度:丹黄刺组(10.49±3.38)mPa·s,参川熟组(11.02±3.13)mPa·s,参山杜组(12.60±3.91)mPa·s,参银生组(15.69±8.02)mPa·s;腓肠肌质量:丹黄刺组(1.083±0.109)g,参川熟组(1.133±0.153)g,参山杜组(1.085±0.116)g,参银生组(1.031±0.257)g,单纯尾吊组(0.999±0.128)g,P<0.05～0.001]。结论:中药复方对模拟失重导致的血循环状态紊乱、骨丢失及肌肉萎缩具有防护作用。
AIM： To investigate the role of Chinese herb decoctions in ameliorating the abnormity of hemorheology, osteoporosis and muscle atrophy in rats under simulated weightlessness.
METHODS： The experiment was conducted in the Fifth Room of Weightlessness Physiology Laboratory, Institute of Space MedicoEngineering between May 2000 and May 2002. 120 healthy male SD rats were selected and randomly divided into 6 groups： control group; tailhanging group [the rats were simulated by head down tilts （HDT） -30° model]; danhuangci decoction group （decoction of danshen, huangqi, ciwujia）; shenchuanshu decoction group （tail-hanging plus decoction of ginseng, chuanziong, shudi）; shenshandu decoction group （tail-hanging plus decoction of American ginseng, haw, duzhong ） and shenyinsheng decoction group （tail-hanging plus decoction of ginseng, yinxing, shengdi）. On the day 30, 10 mL blood of heart, the muscle and bone were taken to detect ① hemorheological parameters： whole blood viscosity, red blood cell deformation, content of fibrinogen, volume packed cells and morphous of red blood cell.②muscle weight, the type and size of muscle fiber. ③bone biomechanical properties and mineral content. RESULTS： ①HDT resulted in obvious decrease of muscle weight, size of muscle fiber and proportion of type Ⅰ muscle fiber （P 〈 0.05-0.01）, so were the area of cortex, content of bone mineral salt and bone biomechanical indexes （P 〈 0.05-0.001）; the hemorhelological parameters changed greatly： blood viscosity in low and middle shear, plasma fibrinogen and the rate of erythrocytes with abnormal shape were increased significantly [blood viscosity in middle shear： （8.25±1.70）, （6.44±0.88） mPa per second; blood viscosity in low shear： （25.10±8.93）, （10.87±5.71） mPa per second; P 〈 0.05 or 0.001]; and the maximum index of erythrocyte deformability （DImax） integral deformability index （IDI） and hematocrit （HCT） were decreased significantly [Dlmax： （60.69±1.20）%, （64.73±2.66）%; IDI： （42.31±2.97）%, （45.00±1.80）%, P 〈 0.01-0.001]. ②Chinese herbs groups were effective in improving the hemorheological parameters and some bone biomechanic indexes, and increasing the weight of gastrocnemius under simulated weightlessness [blood viscosity in middle shear： darthuangci group： （4.41±1.01） mPa per second, shenchuanshu group： （6.32±1.14） mPa per second, shenshandu group： （6.68±0.52） mPa per second; blood viscosity in low shear： danhuangci group： （10.94±3.38） mPa per second, shenchuanshu group： （11.02±3.13） mPa per second, shenshandu group： （12.60±3.91） mPa per second, shenyinsheng group （15.69±8.02） mPa per second; weight of gastrocnemius： danhuangci group： （1.083±0.109） g, shenchuanshu group： （1.133±0.153） g, shenshandu group： （1.085±0.116） g, shenyinsheng group （1.031±0.257） g, tail-hanging group （0.999±0.128） g, P 〈 0.05-0.001]. CONCLUSION： Chinese herbs compound can effectively prevent the hemorheological abnormity, bone loss and muscle atrophy caused by simulated weightlessness.
出处
《中国临床康复》
CSCD
北大核心
2006年第31期43-46,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家863计划资助项目(863-2-3-2-4)~~
