摘要
目的:观察生脉散对失血性休克大鼠肝脏细胞液糖皮质激素受体的调节作用。方法:实验于2005-06/10在佳木斯大学基础医学院病理学教研室完成。选择成年雄性Wistar大鼠36只,按随机数字表法分为失血性休克模型组、生脉散加失血性休克组和假手术组3组,每组12只。制备失血性休克模型。生脉散加失血性休克组于放血前1d灌胃给予生脉散1mL,放血前1h加服一次,于放血后4,8h及处死前1h再分别灌胃给予生脉散1mL(生脉散由佳木斯大学附属医院中药局提供,人参、麦冬、五味子按2∶3∶2的比例组成,煎制为含生药1kg/L)。假手术组颈动脉插管后不放血。失血性休克模型组及假手术组灌胃生理盐水,注入量、次数、时间均与生脉散加失血性休克组给药相同。3组动物均于放血12h后快速断头处死,每组中6只参照谭金兴等方法制备肝细胞液,以3H-地塞米松为放射配体,采用放射配体结合法进行检测。制图求解离常数值和糖皮质激素受体的结合容量,同时测定血浆皮质酮浓度。另每组6只采用一点分析法测定肝细胞糖皮质激素受体,结果用受体特异结合量和受体特异结合位点表示。结果:在实验过程中无动物死亡,全部进入结果分析。①失血性休克模型组和生脉散加失血性休克组大鼠血浆中皮质酮含量比假手术组均明显升高[(29.6±4.7),(12.3±4.5)μg/L,t=6.51,P<0.01];[(34.4±3.8),(12.3±4.5)μg/L,t=9.19,P<0.01];失血性休克模型组与生脉散加失血性休克组皮质酮含量差异无显著性(t=1.95;P>0.05)。②失血性休克模型组和生脉散加失血性休克组大鼠肝细胞液糖皮质激素受体解离常数较假手术组均明显增高[(1.21±0.46),(0.59±0.23)nmol/L;t=2.95,P<0.01];[(1.30±0.53),(0.59±0.23)nmol/L;t=3.01,P<0.01];失血性休克模型组与生脉散加失血性休克组差异无显著性(t=0.31,P>0.05)。③失血性休克模型组大鼠肝细胞液糖皮质激素受体结合容量较假手术组明显降低[(352.2±20.1),(519.7±27.1)pmol/g;t=12.16,P<0.01],生脉散加失血性休克组肝细胞液糖皮质激素受体结合容量明显高于失血性休克模型组[(516.9±31.5),(352.2±20.1)pmol/g,t=10.79,P<0.01],与假手术组差异无显著性(t=0.16,P>0.05)。④失血性休克模型组大鼠肝细胞液糖皮质激素受体特异结合量和受体特异结合位点较假手术组明显降低[(526.1±46.7),(978.5±53.3)pmol/g;t=15.64,P<0.01];生脉散加失血性休克组明显高于失血性休克组[(672.6±47.1),(526.1±46.7)pmol/g;t=5.41,P<0.01],但低于假手术组(t=10.57,P<0.01)。结论:生脉散可升高失血性休克大鼠血浆糖皮质激素含量,提高失血性休克大鼠肝细胞糖皮质激素受体结合容量和受体特异结合位点,并增加糖皮质激素受体解离常数。生脉散的抗休克作用与其减轻休克时糖皮质激素受体且不影响血浆糖皮质激素水平,增强糖皮质激素受体功能有关。
AIM： To observe the regulative effects of Shengmai san （SMS） on the glucocorticoid receptor （GR） in hepatic cytosol of hemorrhagic shock （HS） rats.
METHODS： The experiment was conducted in the Department of Pathology, Jiamusi University Basic Medical College from June to October in 2005. Thirty-six adult male Wistar rats were divided into three groups randomly： sham operated group, HS group and HS plus SMS group, each con taining 12 rats. HS model was prepared, The rats of HS plus SMS group were intragastrically administrated with 1 mL SMS at 1 day and 1 hour before bloodletting, and 4 hours, 8 hours after bloodletting as well as 1 hours before execution （SMS was provided by Bureau of Traditional Chinese Medicine, Jiamusi University Affiliated Hospital, and consisted of ginseng, ophiopogonis tuber and Chinese magnolivine fruit in the proportion of 2：3：2, including 1 kg/L crude drug per decoction）. Sham operated group were not exanguinated after carotid arterial cannula. Sham operated group and HS group were treated with intragastric administration of isotonic Na chloride at the same injection dose, frequency and times as those of SMS plus HS group. Rats of three groups were executed after 12 hours of bloodletting. Six rats of each group were selected to prepare the hepatic cytosol according to Tan Jin-xing＇s method, . and the binding capacity of GR in the hepatic cytosol were measured by radioligand binding assay, using 3H-Dex as the ligand. At the same time, plasma corticosterone concentration （CC） was measured while dissociation constant was measured by charting. Other six rats of each group were selected to assay the GR in the hepatic ceils, and the result was expressed by receptor specific binding capacity and binding site.
RESULTS： All the experimental animals were involved into the result analysis without any drop. ①CC in HS group and SMS plus HS group were obviously higher than that of sham operated group [（29.6 ±4.7）, （12.3 ±4.5） μg/L, t=6.51, P 〈 0.01];[（34.4±3.8）, （12.3±4.5） μg/L, t=9.19, P 〈 0.01], and the difference of CC was not significant between SMS plus HS group and HS model group （t=1.95, P 〉 0.05）.②The dissociation constant of GR in HS group and SMS plus HS group were also obviously higher than that of sham operated group [（1.21±0.46）, （0.59±0.23） nmol/L; t=2.95, P 〈 0.01];[（1.30±0.53）, （0.59±0.23） nmol/L; t=3.01, P 〈 0.01]. The difference of dissociation constant of GR between HS group and SMS plus HS group was not significant （t=0.31, P 〉 0.05）.③The binding capacity of GR in HS group was obviously lower than that of sham operated group [（352.2 ±20.1）, （519.7 ±27.1） pmol/g; t=12.16, P 〈 0.01], and was obviously lower than that of SMS plus HS group [（516.9±31.5）, （352.2±20.1） pmol/g, t=10.79; P 〈 0.01]. The difference was not significant compared with sham operated group （t=0.16, P 〉 0.05）.④The specific binding capacity and site of GR in the hepatic cytosols were obviously decreased in HS group than in sham operated group [（526.1±46.7）, （978.5±53.3） pmol/g; t=15.64, P 〈 0.01]. Compared with SMS plus HS group, the specific binding capacity and site of GR were obviously decreased in HS group [（672.6±47.1）, （526.1±46.7） pmol/g; t=5.41, P 〈 0.01], but increased in sham operated group （t=10.57, P 〈 0.01）.
CONCLUSION： SMS can heighten glucoeortieoid content in HS rats, elevate the specific binding capacity and site of GR and increase the dissociation eonstant of GR in hepatic eytosol. SMS may be beneficial for the treatment of HS by not affecting the level of plasma CC and increasing the level and function of GR.
出处
《中国临床康复》
CAS
CSCD
北大核心
2006年第31期58-60,共3页
Chinese Journal of Clinical Rehabilitation
