摘要
目的:观察百合科植物大蒜球茎中分离出的一种化合物大蒜素对2型糖尿病大鼠血糖的影响及高血脂对糖代谢的影响。方法:实验于2004-10/2005-02在哈尔滨医科大学第一临床医学院实验动物中心完成。选择健康的雄性Wistar大鼠70只,将大鼠按随机数字表法分成2组:对照组10只,糖尿病造模组60只,糖尿病造模组大鼠以高脂高糖饲料(配方:20%蔗糖、2.5%胆固醇、10%猪油、1.0%胆盐、66.5%基础饲料)诱导4周加链脲佐菌素注射进行造模,空腹血糖≥11.1mmol/L为造模成功。将造模成功糖尿病大鼠56只纳入实验,随机数字表法分为5组,即阳性对照组12只,2型糖尿病模型组、大蒜素60,40,30mg/(kg·d)剂量组各11只。2型糖尿病模型组喂基础饲料;阳性对照组喂高脂高糖饲料加大蒜素60mg/(kg·d);大蒜素60,40,30mg/(kg·d)剂量组喂基础饲料加大蒜素60,40,30mg/(kg·d);连续喂养3周。观察不同剂量大蒜素对2型糖尿病、高脂糖尿病和正常大鼠血糖及尿量的影响。结果:对照组10只全部进入结果分析,糖尿病造模组大鼠56只造模成功,进入结果分析。①高脂高糖饲料喂养4周后,糖尿病2型糖尿病模型组大鼠血脂增高、空腹血糖和胰岛素水平升高,胰岛素敏感指数下降,与空白对照组比较,差异有显著性意义(t=5.63～14.22,P<0.01)。②分组施加大蒜素等干预3周后空白对照组、阳性对照组、2型糖尿病模型组大鼠实验前后血糖无明显变化(t=0.18～1.80,P>0.05);大蒜素60,40,30mg/(kg·d)剂量组实验前后血糖均有所下降(t=9.48,7.70,2.35,P<0.01～0.05);实验后大蒜素60mg/(kg·d)剂量组血糖与阳性对照组比较,差异有非常显著性意义(P<0.01)。③实验后大蒜素60,40,30mg/(kg·d)剂量组糖尿病大鼠的尿量显著降低(P<0.01);阳性对照组大鼠尿量也略有降低,但是实验前后比较差异不大(P>0.05);大蒜素60mg/(kg·d)剂量组大鼠尿量与阳性对照组比较差异有显著性意义(P<0.01)。结论:大蒜素能够降低2型糖尿病大鼠的血糖,降糖效果与给药剂量呈正相关,高血脂影响血糖的代谢。
AIM： To study the effect of garlicin, one compound extracted from Allium sativnm L, on the level of blood sugar of rats with type 2 diabetes mellitus （T2DM） and the effect of hyperlipemia on glycometabolism.
METHODS： The experiment was conducted in the Experimental Animal Center of the First Clinical Medical College of Harbin Medical University between October 2004 and February 2005. Totally 70 healthy male Wistar rats were selected and randomly divided into 2 groups： control group＇（n=10）, T2DM model group （n=60）. The rats in the T2DM model group were fed with high fat and sugar diet （prescription： 20% cane sugar, 2.5% cholesterol, 10% lard, 1.0% bile salt and 66.5% basal diet） for 4 weeks following by injection of streptozotocin to establish the models, which were successfully established if the fasting blood sugar ≥ 11.1 mmol/L. The 56 successful T2DM rat models were randomly divided into five groups： positive control group （n=12）, T2DM model group （n=l 1） and high, middle and low doses garlicin groups （n=11, respectively）.The T2DM model group was fed with basal diet; the positive control group was fed with high lipid and sugar food and 60 mg/kg per day garlicin; the three doses garlicin groups were fed with basal diet and 60, 40 and 30 mg/kg per day garlicin, respectively. All the rats were fed for 3 weeks. The effect of garlicin at different doses on the blood sugar and urine volume of T2DM, high fat DM, and normal rats was observed.
RESULTS： All the 10 rats in the control group and 56 T2DM model rats were involved in the result analysis. ①Four weeks later after fed with high fat and sugar diet, the blood fat, fasting blood sugar and insulin levels in the T2DM model group were all increased, the insulin sensitive index was decrease, which had significant differences compared with the control group （t=5.63-14.22, P 〈 0.01）.②After 3 weeks of intervention, the blood sugar in the control group, positive control group and T2DM model group had no obvious change before and after experiment （t=0.18-1.80, P 〉 0.01- 0.05）; while the sugar level was decrease in the three doses garlicin groups （t=9.48, 7.70, 2.35, P 〈 0.01-0.05）; there were significant differences in the sugarAevel between the 60 mg/kg garlicin group and the positive control group （P 〈 0.01）.③The urine volume of the three dosesgarlicin groups after experiment was obviously decreased compared with that before experiment （P 〈 0.01）, so was the positive control group, which had no significant difference before and after experiment （P 〉 0.05）; significant differences were found in the 60 mg/kg garlicin group and the positive control group （P 〈 0.01）.
CONCLUSION： Garlicin can decrease the blood sugar level in T2DM rats, and this effect is positively related to dose of administration. High lipid can influence glycometabolism.
出处
《中国临床康复》
CAS
CSCD
北大核心
2006年第31期73-75,共3页
Chinese Journal of Clinical Rehabilitation
