摘要
目的:研究大孔吸附树脂纯化酸枣仁总皂苷的工艺条件及参数。方法:以酸枣仁总皂苷的洗脱率、精制度等为指标,用蒸馏水、30%乙醇、70%乙醇各3BV(柱床沉降体积)依次洗脱,考察大孔吸附树脂对酸枣仁总皂苷的吸附性能和洗脱参数。结果:12·7ml酸枣仁总皂苷样品液上大孔吸附树脂柱,酸枣仁总皂苷富集于70%乙醇洗脱液部位,洗脱率为85·2%,精制度为208·4%。结论:通过大孔吸附树脂富集与纯化,可较好地纯化酸枣仁总皂苷。
OBJECTIVE： To study the technological parameters of the purification process of total saponins of Semen Ziziphi Spinosae（TSSZS） with macroporous adsorption resin.METHODS：The adsorptive characteristics and elutive parameters of the process of TSSZS were studied by taking the elutive and purified ratio of saponins as markers and washed with 3 bed volume （BV） of distilled water,eluted with aBV of 30% ethanol and aBV of 70% ethanol .RESULTS： 12.7ml of the extractive of TSSZS was purified with a colunm of macroporous adsorption resin and most of saponins were collected in the 70% ethanol, the elutive ratio of saponins was 85.2 % and the purity reached 208.4%. CONCLUSIONS：The process of applying macroporous adsorption resin to adsorb and purify TSSZS is feasible.
出处
《中国药房》
CAS
CSCD
北大核心
2006年第15期1191-1193,共3页
China Pharmacy
关键词
大孔吸附树脂
纯化
酸枣仁
总皂苷
Macroporous adsorption resin
Purification
Semen Ziziphi Spinosae
Total saponins
