摘要
目的:观察激光损伤后不同浓度碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)对RPE细胞修复的作用。方法:采用甲基噻唑基四唑(Methylthiazolyte-trazolium,MTT)方法测定100mW功率的激光损伤后分别应用6种浓度的bFGF,不同时间RPE细胞的损伤修复情况。结果:激光照射后应用bFGF24小时可明显增加RPE细胞的再生,并呈剂量相关。结论:激光照射后应用bFGF对RPE细胞修复有促进作用。
Objective： Observe the effects of bFGF on repair of the cultured bovine retinal pigment epithelium cells after laser photocoagulation in Vitro. Methods： After photocoagulation with 100mW laser, different concentration of bFGF were added and the cell killed in grates（CKR） were measured by MTT assay. Results： The CKRs have a positive linearity relationship with the bFGF concentration in 24 hours group. Conclusion： bFGF can promote the repair of the cultured bovine retinal pigment epithelium cells after laser photocoagulation in vitro.
出处
《中国伤残医学》
2006年第2期18-21,共4页
Chinese Journal of Trauma and Disability Medicine
基金
"九五"军队医药卫生科研基金资助项目
批准号96M143
