摘要
对胃癌组织中结直肠癌缺失基因（ＤＣＣ）腺瘤性息肉病基因／结直肠癌突变基因（ＡＰＣ／ＭＣＣ）基因杂合性缺失（ＬＯＨ）进行分析，以探讨其在胃癌发生发展中的作用。采用ＰＣＲ－ＲＦＬＰ技术，对４５例手术切除胃癌组织ＤＣＣ、ＡＰＣ／ＭＣＣ基因ＬＯＨ进行检测。结果表明ＤＣＣ基因ＬＯＨ率为３３．３％（１５／４５）、ＡＰＣ／ＭＣＣ为３０．０％（９／３０），ＬＯＨ率在肠型及胃型胃癌中无明显差异，与肿瘤大小、组织学分类、浸润深度、淋巴结转移无关。ＤＣＣ基因ＬＯＨ率在Ⅲ、Ⅳ期胃癌组（４８．０％）显著高于Ⅰ、Ⅱ期组（１５．０％）（Ｐ＜０．０５）．ＡＰＣ／ＭＣＣ基因ＬＯＨ在早期及进展期均可见到，与ＤＣＣ基因ＬＯＨ无相关性。结果提示ＤＣＣ、ＡＰＣ／ＭＣＣ基因ＬＯＨ对两型胃癌的发生发展均有一定作用。ＡＰＣ／ＭＣＣ基因ＬＯＨ在胃癌的早期发生及发展中起作用，而ＤＣＣ基因ＬＯＨ则是晚期改变，可能与临床预后相关。
In order to evaluate the role of DCC and APC/MCC genes alterations in the development and progress of gastric cancer, we investigated the loss of heterozygosity(LOH) at the DCC and APC/MCC genetic loci in 45 surgical specimens of gastric cancer using the polymerase chain reaction(PCR) and restriction fragment length polymorphism analysis(RFLP). LOH was detected in 9/30(30.0%) of informative cancers at APC/MCC and in 15/45(33.3%) at DCC. LOH at the APC/MCC and DCC was found in both intestinal and gastric types of gastric cancer, and was not correlated to histological types,tumor size,invasion and lymphnode metastases. However,the incidence of LOH at DCC locus in stages Ⅲ and Ⅳ(48.0%)was significantly higher than that in stages Ⅰ and Ⅱ (15.0%,P<0.05). LOH at APC/MCC locus was found in early stage and in advanced stage as well. There was no significant correlation between LOH at DCC and LOH at APC/MCC(P>0. 05). These results suggest that alteration of DCC and APC/MCC genes may be involved in the development and progress of the two types of gastric cancer.LOH at APC/MCC locus plays an important role at the early stage of carcinogenesis and progress, while LOH at DCC locus occurs at a later stage and may be correlated with prognosis of patiets.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1996年第5期269-272,共4页
Chinese Journal of Medical Genetics
基金
国家自然科学基金
关键词
胃肿瘤
杂合性缺失
基因缺失
Gastric cancer DCC gene APC/MCC gene Loss of heterozygosity
