摘要
目的：观察回阳生肌膏水、醇提取物对人慢性皮肤溃疡创缘成纤维细胞（uHFB）增殖的影响，并探讨其对c—fos、c—mvc表达的影响。方法：组织块法培养uHFB；alarmarblue摄入法检测回阳生肌膏水、醇提取物对uHFB的增殖作用；免疫细胞化学方法检测二者对c—fos、c—myc表达的影响。结果：与对照组比，回阳生肌膏醇、水提取物分别在0．027～0．213mg／L和0．15．1．2mg／L浓度范围内对uHFB有促增殖作用（P＜0．05或P＜0．01）；醇提物组c—fos蛋白在2h达峰值，水提物组c—k蛋白在1—3h内持续增加至醇提物组2h时水平；水、醇提物组c—myc蛋白在2—3h内均增加，但醇提物组的表达强度高于水提物组。结论：回阳生肌膏可能通过上调核转录因子表达正向调控修复细胞增殖，加速创面愈合。
Objective To observe the effects of alcohol and aqueous extracts from Huiyang Shengji Gao（HYSJG） on ulcer human fibroblast（uHFB） proliferation and to evaluate the promoting-healing function of HYSJG Methods Using explant culture techniques, we obtained populations of ulcer marginal fibroblast （uHFB）. Alarmar blue assay was used for observing cell proliferation, and immunocytochemistry for c-fos and c-myc expression. Results For alcohol ex- tract, obvious proliferation-promoting concentrations were 0.027 - 0.213 mg/L （P 〈 0.05 or P 〈 0.01 ）. For aqueous extract, they were 0.15 - 1.2 mg/L （ P 〈 0.05 or P 〈 0.01 ）. After uHFB cultured with alcohol extract for 2 hours, c- fos expression of uHFB came to max. From hour 1 to hour 3, c-fos expression of uHFB in aqueous extract group increased up to the level of alcohol extract group at hour 2. The two extracts could both increase c-myc expression from hour 2 to hour 3, but alcohol extract group had more expression intensity. Conclusion Extracts of HYSJG can promote uHFB proliferation, and upward regulate c-fos and c-myc expressions, suggesting that HYSJG can promote healing cells proliferation to accelerate wound healing by upward regulation of nuclear transduction factors.
出处
《中国中西医结合外科杂志》
CAS
2006年第4期391-394,共4页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
