摘要
在装备了二极管阵列检测器(PDA)的高压液相色谱(HPLC)上,应用C30柱,番茄红素几何异构体获得了良好的分离。色谱条件为:WatersYMCCarotenoidS-5(4.6×250mm)柱;乙腈-甲醇-三乙胺(75∶25∶0.05,V/V)为流动相A,甲基叔丁基醚-三乙胺(100∶0.05,V/V)为流动相B,流动相B在8min内由0%线性增至55%,而后维持恒定至35min;流速:1.0mL/min;PDA波长范围:260～700nm;进样量:20μL。根据各组分的色谱行为、光谱特征和在碘催化下发生几何异构的产物分析,13,13'-顺式番茄红素、11,11'-顺式番茄红素、9,9'-顺式番茄红素和全反式番茄红素4个组分被鉴定。本项研究的结果奠定了应用C30-HPLC-PDA定量检测番茄红素几何异构体的基础。
The lycopene geometrical isomers were readily separated by HPLC equipped with photodiode array detector on a C30 column under following HPLC conditions： YMC^TM Carotenoid S-5 column （250 ×4.6mm）, mobile phase A and B were acetonitrile-methanol-TCA（75：25：0.05, V/V） and MTBE-TCA（100：0.05, V/V）, respectively. Mobile phase B increased linearly from 0% to 55%（V/V） in the first 8 minutes and then kept constant at the flow rate of 1.0mL/min. Sample injection volume was 20μL. According to its chromatographic behavior, spectral characteristics and geometrical transition catalyzed by iodine, 4 isomers of 13,13＇Z-, 11,11＇Z-, 9,9＇Z- and all Eisomers were identified. Result from this study formed the base to quantify lycopene geometrical isomers by C30-HPLC-PDA.
出处
《食品工业科技》
CAS
CSCD
北大核心
2006年第7期49-51,54,共4页
Science and Technology of Food Industry
基金
国家"十五"食品安全重大科技专项课题(2001BA804A)。
