摘要
目的观察金匮肾气丸对192Ir近距离照射大鼠脊髓后细胞凋亡的影响。方法将120只SD大鼠随机分成为模型组、金匮肾气丸组、泼尼松组和正常组,各组每日给予相应的药物,连续给药14天后,除正常组外,其它各组均采用后装技术在大鼠脊髓进行192Ir近距离照射,照射剂量均为22Gy,大鼠分别在照射后8h、24h、4周时取损伤节段脊髓,应用HE、TUNEL染色,光镜和电镜观察。结果近距离照射各组大鼠脊髓后8h、24h时HE染色均未观察到明显组织结构上的改变,4周时脊髓白质区出现组织疏松和出血等病理改变。TUNEL染色和电镜显示:与模型组比较,金匮肾气丸组和泼尼松组照射后8h大鼠脊髓的细胞凋亡指数显著下降(P<0·01),而在照射后24h、4周时间点则差异无显著性。结论金匮肾气丸具有肾上腺皮质激素样作用,可对抗大鼠近距离放射性损伤早期胶质细胞的凋亡作用。
Objective To study the effect of Jingui Shenqi Pill （JSP） on morphology of spinal cell apoptosis in rats injured by 192Ir irradiation. Methods One hundred and twenty rats were randomly divided into four groups： the model group, the JSP group, the prednisone group and the normal group. Corresponding pharmaceutics were given to rats once a day for 14 days respectively. Then except rats in the normal group, the others received 192Ir interstitial irradiation with the dosage of 22 Gy using back-fixing technology. The injured segments of spinal cord were taken out for HE staining, TUNEL examination and observation with electron microscope 8 hrs, 24 hrs and 4 weeks after irradiation. Results HE staining examination showed no obvious histological change in rats 8 and 24 hrs after irradiation, but pathological changes, as tissue rarefaction and hemorrhage did found in white matter of spinal cord shown by TUNEL 4 weeks later. Electron microscopic examination and TUNEL staining showed that as compared with the model group, the apoptotic index in the JSP and predinisone treated groups was significantly lower （P 〈 0.01） 8 hrs after radiation, but it showed insignificant difference between groups at the time points of 24 hrs and 4 weeks after radiation （P 〉 0.05）. Conclusion JSP could act against apoptosis of gliocyte in spinal cord of rats in early stage after brachytherapy, indicating that JSP possessing a prednisone-like action.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2006年第7期633-635,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
浙江省自然科学基金资助课题(No.302606)
关键词
金匮肾气丸
脊髓
放射性损伤
细胞凋亡
Jingui Shenqi Pill
spinal cord
radiation injury
apoptosis
