摘要
目的探讨川芎嗪注射液治疗局灶性脑缺血/再灌注损伤的时间窗。方法40只SD雄性大鼠,随机分为5组(n=8),对照组(即生理盐水组)和川芎嗪注射液治疗组(T1、T2、T4和T6组),分别于缺血/再灌注后1h、2h、4h和6h腹腔注射川芎嗪注射液(20mg/kg),每24h一次,共3次。采用右侧颈内动脉单丝尼龙线栓塞致大脑中动脉阻闭(MCAO)120min建立局灶性脑缺血模型。再灌注后72h,评估神经功能缺陷评分(NDS)并处死动物,取大脑行2,3,5-氯化三苯四唑(TTC)染色,以测量脑梗死容积。结果再灌注后72h测NDS,对照组明显高于T1组(P=0.001)、T2组(P=0.005)和T4组(P=0.002),T1、T2、T4和T6组间NDS无差异。再灌注后72h,对照组的脑梗死容积(205±72mm3)明显大于T1组(116±44mm3,P=0.001)、T2组(127±30mm3,P=0.003)和T4组(135±35mm3,P=0.007);在T1和T6组间也有统计学差异(P=0.035);其余各组的脑梗死容积无明显差异。结论川芎嗪治疗大鼠短暂局灶性脑缺血/再灌注损伤的有效治疗时间窗不宜超过4h。
Objective To investigate the therapeutic time window of tetramethylpyrazine injection for transient focal cerebral ischemic/reperfusion injury. Methods Forty male SD rats, weighing 280 - 320 g, were randomized into five groups （n = 8 each group ）： control group received intraperitoneally normal saline; therapeutic groups of TI, T2, T4 and T6 received intraperitoneally tetramethylpyrazine injection （20 mg/kg） at 1 h, 2 h, 4 h and 6 h respectively after 120 min of middle cerebral artery occlusion （MCAO） with 3-0 nylon monofilament. The neurological outcome was evaluated at 72 h after reperfusion. The brain infarct volume was determined by 2, 3, 5-triphenyltetrazolium chloride （TIC） staining. Results The neurological deficit scores （NDS） for group TI （P =0.001）, group T2 （P =0.005） and group T4 （P =0.002） 72 h after the reperfusion were significantly lower than that of the control group. There was no significant difference among groups TI, T2, T4 and T6 for NDS. The infarct volume of the control group （205 ±72 mm^3） was significantly greater than those of group T1 （ 116 ± 44 mm^3, P = 0. 001 ）, group T2 （ 127 ± 30 mm^3, P = 0. 003 ） and group T4 （ 135 ± 35 mm^3, P=0.007） respectively 72 h after the reperfusion. There was also significant difference between group T1 and group T6 for infarct volume. No difference was found among groups T1, T2 and T4 for infarct volume. Conclusion Therapeutic time window for tetramethylpyrazine solution in transient focal cerebral ischemic/reperfusion injury should be less than 4 h.
出处
《中华神经外科疾病研究杂志》
CAS
2006年第4期334-337,共4页
Chinese Journal of Neurosurgical Disease Research
基金
国家自然科学基金资助项目(30371763)
关键词
川芎嗪
脑缺血
再灌注
治疗时间窗
Tetramethylpyrazine
Cerebral ischemia
Reperfusion
Therapeutic time window
