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毛细管电泳用于Y染色体微缺失的研究 被引量：2

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摘要 目的:探讨毛细管电泳在研究男性不育中特发性无精子症和射出精液严重少精子症与Y染色体无精子因子(azoospermiafactor,AZF)缺失的作用。方法:应用多重PCR技术对4例无精子症和29例严重少精子症患者的外周血细胞中Y染色体AZF所在的11.23区的15个位点进行扩增,分别用琼脂糖凝胶电泳和毛细管电泳分离扩增产物,并以6例正常生育男性和2例女性为对照。结果:毛细管电泳发现4例无精子症中3例发生缺失,29例严重少精子症患者中6例发生缺失。其中6例同为SY254(C)、SY242(C)、SY255(C)、SY239(C)、SY152(D)缺失。6例正常生育男性毛细管电泳未发现Y染色体微缺失,而凝胶电泳有2个条带模糊,难以判断。结论:毛细管电泳可提高AZF检测准确性。Y染色体AZFc/DAZ的缺失可能是引起无精子和严重少精子并造成男性不育的重要原因之一。
作者 刘圣英 刘雨生 童先宏 郭通航 宋影 周桂香
机构地区 安徽省铜陵市第四人民医院 安徽省立医院生殖中心
出处 《生殖与避孕》 CAS CSCD 北大核心 2006年第8期504-508,共5页 Reproduction and Contraception
关键词 男性不育 Y染色体 无精子因子 毛细管电泳
分类号 R698.2 [医药卫生—泌尿科学]
  • 相关文献

参考文献13

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二级参考文献26

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共引文献69

同被引文献14

  • 1蔡志明,肖晓素,刘晓翌,王勇强.无精症、严重少精症患者Y染色体无精子因子微缺失的遗传学研究[J].中华医学遗传学杂志,2005,22(1):85-87. 被引量：16
  • 2于海涛,岳丽玲.Y染色体微缺失与男性不育的研究现状[J].中国优生与遗传杂志,2006,14(2):1-2. 被引量：14
  • 3周友泉,王厚照,刘芳,史彩虹,史化民.男性不育患者Y染色体AZF区域微缺失的STS位点分析[J].实用医技杂志,2006,13(14):2432-2434. 被引量：5
  • 4胡兰萍,刘能辉,潘乾,梁德生,龙志高,胡浩,朱海燕,戴和平,蔡芳,邬玲仟,夏昆,夏家辉.原发性无精、少弱精症患者Y染色体AZF微缺失检测[J].中南大学学报（医学版）,2007,32(2):241-245. 被引量：26
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  • 8[5]Oates BD,Silber S,Brown LG,et.al.Clinical Characterization of 42 oligospermic or azoospermic men with microdelefon of AZFc region of the Y chromosome and of 18 children conceived via ICSI[J].Hum.Reprod,2002,17:2813-24.
  • 9[9]Hopps C V.Mielnik A.Goldtein M.et al.Detection of spem in men with Y chromosome microdeletions of the AZFa.AZFb and AZFc regions[J].Hum Reprod,2003,18(81):1660-1665.
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引证文献2

二级引证文献4

生殖与避孕
2006年 第8期

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