摘要
目的:β-连环蛋白是一种多功能、广泛分布的蛋白,参与了包括胚胎形成及个体发育等许多生物过程,近年来在生物及医学领域受到广泛关注。本文就β-连环蛋白在皮肤干细胞选择性分化的作用进行综述。资料来源:应用计算机检索PUBMED1996-01/2005-01期间的相关文章,检索词为“beta-catenin,epidermalstemcell,differentiation”,并限定文章语言种类为English。同时计算机检索万方数据库1996-01/2005-01期间的相关文章,检索词为“β-连环蛋白,皮肤干细胞,分化”,并限定文章语言种类为中文。资料选择:对资料进行初审,并查看每篇文献后的引文。纳入标准:文章所述内容应与β-连环蛋白在皮肤干细胞选择性分化研究相关。排除标准:重复研究或Meta分析类文章。资料提炼:共收集到22篇符合纳入标准的文献,涉及β-连环蛋白的生物学特性、皮肤干细胞的分布、表皮干细胞的分化调控、β-连环蛋白与皮肤干细胞的定向分化、β-连环蛋白功能的可能作用机制等方面。资料综合:β-连环蛋白的生物学功能包括粘合连接与信号传导。粘合连接是指细胞膜上的钙黏蛋白依次与细胞膜中的β-连环蛋白、α-连环蛋白及肌动蛋白相连形成的特殊结构以完成细胞-细胞、细胞-细胞外基质之间的黏附和相互作用。信号传导指的是β-连环蛋白在Wnt信号通路中的核心作用。β-连环蛋白的这两种功能相互依赖、相互影响,对于皮肤干细胞的分化有着特殊的意义:干细胞对基底膜的黏附是干细胞维持其特性的基本条件,对基底膜的脱黏附是诱导干细胞脱离干细胞群落,进入分化周期的重要调控机制之一。表皮干细胞的分化受内源性和外源性调控,整合素在表皮基底层干细胞与基底膜的信息调控和黏附中起重要作用。β-连环蛋白作为一种细胞因子,存在于表皮干细胞膜表面,在皮肤干细胞分化途径的选择及定向迁移中发挥着重要作用。结论:β-连环蛋白近年来在生物及医学领域受到广泛关注的原因主要与其粘合连接、信号传导两大功能有关。体内参与调控表皮干细胞与毛囊干细胞分化的因子有多种,β-连环蛋白与它们共同形成一个复杂的网络,为表皮细胞的分化提供了重要的微环境。
OBJECTIVE： β-catenin is an ubiquitously distributed protein with multiple functions, which is crucially involved in a variety of biological processes including embryonic formation and individual development. Recent years, it has caught more attention from biological and medical fields. In this paper, we summarize the important roles of βcatenin in the process of selective differentiation of epidermal stem cell.
DATA SOURCES： A computer-based online search of PUBMED was undertaken for related articles published in English between January 1996 and January 2005 with the key words of ＂beta-catenin, epidermal stem cell, differentiation＂. Meanwhile, we searched wanfang database for related articles published in Chinese between January 1996 and January 2005 with the same key words in Chinese.
STUDY SELECTION： The data were selected firstly, and the references of each article were looked up. Inclusive criteria： Articles about β-catenin and selective differentiation of epidermal stem cell. Exclusive criteria： Repetitive studies or Meta analysis.
DATA EXTRACTION： A total of 22 literatures met the inclusive criteria were collected, involving in biological features of β-catenin, distribution of epidermal stem cell, differentiated regulation of epidermal stem cell, β- catenin and selective differentiation of epidermal stem cell and possible mechanism of β-catenin function.
DATA SYNTHESIS： The biological functions of β-catenin include adhesion connection and signal conduction. Adhesion connection refers to the special structure by connection of cadherin on cellular membrane with β-catenin, α-catenin and actin by turns, which can finish the adhesion between cell to cell and cell to excellular matrix and their interaction. Signal conduction refers to the important role of β-catenin in Wnt signal pathway. These two functions of β-catenin are dependent and interacted each other, which has special significance in differentiation of epidermal stem cell： The adhesion of stem cell to basal membrane is the basic condition for stem cell to maintain its characteristics, and its deadhesion is one of the important regulative mechanisms to induce the stem cell detach the stem cell community to enter differentiation stage. Differentiation of epidermal stem cell is modulated by endogenous and exogenous, and integrin displays an important role in the information regulation and adhesion of stem cell of epidermal basal layer and basal membrane. β- catenin as a kind of cell factor, exists in the surface of epidermal stem cells, and plays a significant role in selection of differentiated approaches and directional migration of epidermal stem cells.
CONCLUSION： In recent years, β-catenin has caught more attention from biological and medical fields, that is because its two functions： adhesion connection and signal conduction. There are many factors participating in regulating epidermal stem cell and differentiation of follicular stem cell, which accompanying β-catenin forms a complex network to provide important microenvironment for differentiation of epidermal stem cells.
出处
《中国临床康复》
CSCD
北大核心
2006年第33期122-124,共3页
Chinese Journal of Clinical Rehabilitation
基金
北京市自然科学基金资助项目(7042047)~~
