摘要
钩吻碱类提取物在浓度大于１．２５μｇ／ｍｌ以上时对混合淋巴细胞培养反应（ＭＬＲ）均有不同程度的抑制作用，具有统计学意义（Ｐ＜０．０５）的最低抑制浓度为２．５μｇ／ｍｌ，抑制率为１８．３％。单独采用Ｃ５７ＢＬ／６ｊ小鼠脾细胞进行实验，促有丝分裂剂为细菌脂多糟（ＬＰＳ５μｇ／ｍｌ），在钩吻碱类提取物浓度为４０μｇ／ｍｌ才出现抑制作用（Ｐ＜０．０５），抑制率为１８％，改用刀豆蛋白Ａ（ＣｏｎＡ２μｇ／ｍｌ）刺激，钩吻碱类提取物最低抑制浓度为２０μｇ／ｍｌ，抑制率为１２．４％（Ｐ＜０．０５）．脾细胞先经ＣｏｎＡ活化．后用白细胞介素２（５ｕ／ｍｌ）维持培养，此时钩吻碱类提取物的最低抑制浓度为４０μｇ／ｍｌ．抑制率为１７．１％（Ｐ＜０．０５）。
Gelsemium alkaloids abstract(GAA)in concentrations of more than 1.25μg/ml could,tosome extent,inhibit mixed lymphocyte culture response. The minimal inhibitory concentration(MIC)ofstatistical significance(P<0.05)was 2.5μg/ml,with an inhibitory rate of 18.3%,When C57 BL/6j motuse splenocytes were used,and the proliferation was induced by lipopolysaccharide(LPS 5μg/ml),the MIC ofGAA was 40μg/ml(P<0.05)with an inhibitory rate of 18%,If concanavalin A(Con A 2μg/ml)is used ,the MIC of GAA was 20μg/ml(p<0.05) with an inhibitory rate of 12.4%,Splenocytes were activatedfirstly by Con A and then the culture was maintained in the precence of interleukin 2(IL-2).Under thiscondition,the MIC of GAA was 40μg/ml(P<0.05),with an inhibitory rate of 17.1%.
出处
《第一军医大学学报》
CSCD
1996年第2期74-75,共2页
Journal of First Military Medical University
关键词
钩吻
生物碱
脾细胞
增殖反应
Gelsemium elegans benth
alkaloid
splenocyte
proliferation
