摘要
以非洲菊试管苗叶柄切段和幼花托为外植体，进行芽诱导分化培养比较试验。结果表明，MS＋6-BA4．0mg／L＋IBA 1．0mg／L培养基较适合试管苗叶柄切段的诱导出芽，出芽率为30．0％～36．9％；接种后15～19d，试管苗叶柄切段的出芽率为30．0％～42．2％，污染率为0～14．7%；幼花托的诱导出芽率为0～23．1%，污染率为23．1%～69．2%，与叶柄切段差异明显。由此可见，不同外植体对诱导分化出芽的效果差异很大，以试管苗叶柄切段为外植体进行诱导分化，可缩短诱导周期．提高诱导出芽率，降低污染程度，可作为非洲菊组培快繁生产中诱导出芽的新途径。
Petiole fragments and young receptacle derived from test-tube cultured flameray gerbera were used as explants, the induction and differentiation culture of buds experiments were conducted. The results showed that the medium MS-F6-BA 4. 0mg/L-FIBA 1.0mg/L was suitable to induce buds from petiole, up to 30. 0%-36. 0% of induction rate. After inoculated for 15-90 days, the buds rate of petiole fragments and young receptacle was 30.3%-42.2% and 0-23.1 %, respectively. In contrast, the contaminated rate was 0-14. 7% and 23.1%-69.2%, respectively. The effect on the induction and differentiation buds by different explants was very different. Using petiole fragments from test-tube plantlet as the explants to induce buds could shorten the induction period, increase the buds rate and decrease the contamination, and be recommended as a new way to induce buds in tissue culture and rapid propagation production of flameray gerbera.
出处
《广西农业科学》
CSCD
2006年第4期355-357,共3页
Guangxi Agricultural Sciences
关键词
非洲菊
叶柄切段
幼花托
诱导分化
出芽率
污染率
flameray gerbera
petiole fragments
young receptacle
induction and differentiation
buds rate
contamination rate
