摘要
目的建立兔脊髓损伤(SCI)后体位性低血压(OH)动物模型,探讨斜床站立训练和生脉饮对兔SCI后OH的影响。方法应用胸5脊髓完全横断和斜床抬高60°的方法建立兔SCI后OH模型,各组动物分别于术后第4天开始斜床站立训练和/或服用生脉饮,共治疗28d。用无创兔耳血压仪检测各组动物术后3d、10d、17d、24d、31d平卧位和60°体位收缩压(SBP)和脉率(PR)变化,用高压液相色谱-电化学法检测术后31d平卧位和60°体位血浆肾上腺素(E)和去甲肾上腺素(NE)浓度变化。结果联合治疗组术后24d60°体位收缩压高于损伤组,60°体位较平卧位收缩压平均下降值明显低于损伤组。术后31d训练组、联合治疗组60°体位收缩压高于损伤组,生脉饮组、训练组、联合治疗组60°体位收缩压较平卧位收缩压平均下降值均显著低于损伤组;损伤组、生脉饮组、训练组及联合治疗组平卧位和60°体位血浆肾上腺素、去甲肾上腺素浓度均低于假手术组,生脉饮组、训练组、联合治疗组平卧位和60°体位下血浆E、NE浓度与损伤组比较无显著性差异。结论斜床站立训练和生脉饮治疗能部分改善兔SCI后OH,联合两种治疗可缩短疗程,但均不影响交感神经活动度。
Objective To develop an experimental model of orthostatic hypotension （OH） after spinal cord injury （SCI） in rabbits and investigate the effect of tilting table exercise and Shengmaiyin on it. Methods Rabbit model of OH after SCI were induced by complete transection of spinal cord at the T5 spinal segment and raised to 60° head up tilt （HUT） on a tilting table. Tilting table exercise and Shengmaiyin were applied to different groups for 28 d from 4 days after operation. Systolic blood pressure （SBP） and pulse rate （PR） were observed with non-invasive sphygmobolometer of rabbit 3, 10, 17, 24, 31 d after the operation. Liquid chromatography with electrochemical detection（HPLC-ECD） was used to detect the concentrations of plasma norepinephrine （NE） and epinephrine（E） with horizontal position and HUT 60° on the 31st day. Results SBP with HUT 60° in rabbits of combined treatment group was significantly higher than that in rabbits of SCI group on the 24th day after operation,and the drop in SBP from horizontal position to HUT60° was significantly lower than that in rabbits of SCI group. SBP with HUT 60° in rabbits of exercise group and combined treatment group was significantly higher than that in rabbits of SCI group on the 31st day after operation. The drop in SBP from horizontal position to HUT 60° was significantly lower in rabbits of Shengmaiyin group, exercise group and combined treatment group than that in rabbits of SCI group on the 31st day after operation. The concentration of E and NE of SCI group, Shengmaiyin group, exercise group and combined treatment group was lower than that of sham group, but there was no significant difference among these groups. Conclusion The treatment with tilting table exercise and Shengmaiyin can improve OH after SCI partially in rabbits without involving norepinephrine and epinephrine significantly. The period of treatment can be shorten when both therapies used together.
出处
《中国康复理论与实践》
CSCD
2006年第7期565-567,共3页
Chinese Journal of Rehabilitation Theory and Practice
关键词
斜床站立训练
生脉饮
体位性低血压
脊髓损伤
兔
tilting table exercise
Shengmaiyin
orthostatic hypotension
spinal cord injury （SCI）
rabbit
