摘要
利用固定化果胶酶对四种不同果汁澄清条件及效果进行研究,结果表明,固定化果胶酶澄清四种不同果汁的效果明显,其中澄清桔汁的果胶酶重复使用20次以上,酶活力及透光率仍可维持在80%以上,其最适反应条件是:果汁浓度50%;pH3.0~3.5;温度45~50℃;反应时间2小时;酶量每毫升果汁0.05g固定化果胶酶;澄清时间20小时。
The conditions and effects of clarifying four kinds of fruit juices with im-mobilized pectinase were studied.The results showed that the immobilized pectinase hasa distinct clarification effect on citrus,apple,pear and plum juices. Both emzyme activityand pervious efficiency to light retained up to 80%after reacting more than 20 timeswith immobilized pectinase in citrus juice. The optimum reaction conditions of citrusjuice were as follows:juice concentration 50%,pH 3.0~3.5,reaction temperature 45~50℃,reaction time 2h,immobilized pectinase dosage 0.05 g/ml juice,clarification time20h.
关键词
固定化果胶酶
果汁
澄清
果胶酶
透光率
Immobilized pectinase,Fruit juice,Pervious efficiency to light