摘要
目的观察单纯控制食谱、控制食谱联合有氧运动或降脂药对实验性肥胖大鼠血脂和血管内皮功能的影响。方法Wistar大鼠随机分为：F组：饲予高脂饲料；N组：饲予基础饲料；A组：高脂饲养8周后予单纯控制食谱12周,B组：高脂饲养8周后予控制食谱联合游泳12周,C组：高脂饲养8周后予控制食谱联合藻酸双酯钠12周。干预12周后,处死全部大鼠,测定Lee指数；血脂指标：总胆固醇(TC)、甘油三酯(TG)；血管内皮因子：血浆内皮素(ET)、血清一氧化氮(NO),血浆血管性假血友病因子(vWF),免疫组化法检测腹主动脉细胞间黏附分子1(ICAM-1)蛋白的表达,逆转录-聚合酶链反应(RT-PCR)法检测腹主动脉ICAM-1 mRNA的表达。结果A组的TC、TG、ET低于F组(均尸＜0．05)；B、C组的Lee指数、TC、TG、ET、vWF、ICAM—1蛋白和mRNA表达水平均低于F组(均P＜0．05)；A、C两组NO均高于F组(均P＜0．05),与N组比较差异无统计学意义(均P＞0．05),B组NO同时高于F组和N组(P＜0．01)。结论单纯控制食谱能改善血脂和血管舒缩功能,控制食谱联合有氧运动或联合降脂药藻酸双酯钠在减重、降脂和改善血管舒缩、凝血、黏附功能方面均有效,而控制食谱联合有氧运动改善舒缩功能的效果更为显著,因此是单纯肥胖早期血管内皮功能障碍的最佳干预选择。
Objective To observe the effect of diet-control only, diet-control with swimming training or with polysaccharide sulfate （ PSS）, a kind of blood lipid-lowering drug on the serum lipid level and vascular endothelial function in obese rats fed by fat-rich-diet. Methods Forty Wistar rats were randomly divided into the following 5 groups ： group F （ n = 8 ）, group N （ n = 8） , group A （ n = 8 ）, group B （ n = 8） and group C （ n = 8）, where the rats were given fat-rich-diet, basic-diet, 12 weeks of diet-control after 8 weeks of fat-rich-diet, 12 weeks of diet-control with swimming training after 8 weeks of fat-rich-diet and 12 weeks of diet-control with PSS after 8 weeks of fat-rich-diet, respectively. All rats were sacrificed after 12 weeks of intervention. Then the levels of Lee index, serum total cholesterol （TC）, triglyceride （ TG）, plasma endothelin （ ET）, nitric oxide （NO） and von Willebrand Factor（vWF） were measured. The protein expression of intercellular adhesion molecule-1 （ ICAM-1 ） in artery endothelium was evaluated by immunohistochemistry （IHC）and the gene expression of ICAM-1 was examined by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR）. Results After the interventions for 12 weeks, the levels of serum TC, TG and ET decreased in group A（P〈0.05）. The levels of Lee index, TC, TG, ET, vWF, ICAM-1 protein and ICAM-1 mRNA decreased in group B and C （P 〈0. 05）. Three interventions increased serum NO production （P 〈 0. 05 ） in group B. Conclusions Diet-control could a meliorate the hyperlipidemia and vascular function. Diet-control with swimming training and diet-control with PSS could result in weight loss of rats and meliorate the hypedipidemia, vascular endothelial function, coagulatory activities and adhesive dysfunction. The effects of diet-control with swimming on vascular endothelial function were prominent.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2006年第8期607-610,共4页
Chinese Journal of Pediatrics
基金
山东省科委基金(2002BB1DBA3)
关键词
干预性研究
大鼠
肥胖
内皮
血管
Intervention studies
Mice, obese
Endothelium, vascular
