期刊文献+

成人隐匿性自身免疫性糖尿病患者CD4^+CD25^+T细胞的变化研究 被引量:7

Changes of CD4^+CD25^+T cells in patients with Latent autoimmune diabetes in adults
下载PDF
导出
摘要 目的:通过观察成人隐匿性自身免疫性糖尿病(LADA)患者CD4+CD25+T细胞的变化并与速发性1型糖尿病(T1DM)比较,了解成人隐匿性自身免疫性糖尿病患者T细胞免疫功能的变化及与1型糖尿病的异同点。方法:LADA组24例,速发性T1DM18例,对照组20例,应用流式细胞技术测定3组入选者T细胞表面分子CD4、CD8、CD25、CD4+CD25+、CD8+CD25+、CD4+CD25+CD62L+,以百分比表示各表面分子阳性T细胞占外周血淋巴细胞的比例。结果:LADA与T1DM组CD4+T细胞、CD4/CD8比值明显高于健康对照组(P<0.01),LADA与T1DM对比无差异。LADA组CD25+、CD4+CD25+、CD4+CD25+CD62L+T细胞高于健康对照组(P<0.05),明显高于T1DM组(P<0.01)。T1DM组CD25+、CD4+CD25+、CD4+CD25+CD62L+T细胞略低于健康对照组,但无统计学意义(P>0.05)。结论:LADA患者外周血中诱导免疫耐受的CD4+CD25+、CD4+CD25+CD62L+T细胞较对照组升高并明显高于T1DM患者。LADA患者胰岛β细胞功能下降较速发性T1DM患者相对缓慢可能与CD4+CD25+T细胞的免疫保护有关。 Objective:To observe the changes of CD4^+ CD25^+T ceils in patients with Latent autoimmune diabetes in adults. Methods :The lymphocyte subpopulations( CD4^+ ,CD8^+ , CD25^+ ,CD4^+ CD25^+ ,CD8^+ CD25^+, CD4^+ CD25^+ CD62L^+ T cells ) in peripheral blood mononuelear were in 24 LADA, 18 type 1 diabetic with rapid progression, and 20 normal control by flow cytometric. We assessed the immune changes of T cells subsets of LADA and compared with type 1 diabetic with rapid progression. Results : ( 1 ) Patients with LADA and typel diabetes had higher CD4^+ T cells and CD4^- CD8^- ratio compared with patients type 2 diabetes ( P 〈 0. 05). There were no differences between LADA and type 1 diabetes( P 〉 0.05 ). (2)The CD25^+, CD4^+ CD25^+, CD4^+ CD25^+ CD62L^+ T cells in LADA patients was higher than normal control( P 〈 0.05 ), and more higher than type 1 diabetes ( P 〈 0.01 ). Conclusion :The increasement of regulatory T cells may play an important protective role in development of LADA.
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2006年第8期771-774,共4页 Chinese Journal of Immunology
基金 山东省医药卫生科技发展计划项目(199940)
关键词 UADA(成人隐匿性自身免疫性糖尿病) CD4^+CD25^+T细胞 流式细胞术 Latent autoimmune diabetes in adults CD4^+ CD25^+ T regulatory cells Flow cytometric
  • 相关文献

参考文献8

  • 1Camara N O,Ng W F,Hernandes-Fuentes M et al.Human CD4^+ CD25^ + T cells regulate CD8^ + T cells activation[J].Transplant Proc,2002;34(20):2858-2860.
  • 2Millicevic Z,Knezevic J,Sabioncello A et al.B-cell secretory function and CD25^ + lymphocyte subsets in the early stages of type 1 diabetes mellitus[J].Exp Clin Endocrinal Diabetes,2004;112(2):181-186.
  • 3施毕旻,成兴波,於葛华,张学光.1型糖尿病外周血淋巴细胞CD_(28)、4-1BB共刺激分子的表达与胰岛功能的相关性[J].中华内分泌代谢杂志,2003,19(1):48-49. 被引量:4
  • 4Shimada A,Kodama K,Morimoto J et al.Detection of GAD-reactive CD4^+ cells in so-called "type 1B" diabetes[J].Ann N Y Acad Sci,2003;1005(5):378-386.
  • 5Iellem A,Mariani M,Lang R et al.Unique chemotactic response profile and specific expression of chemokine receptors CCR4 and CCR8 by CD4^ + CD25^ + regulatory T cells[J].J Exp Med,2001 ;194(6):847-885.
  • 6郑峻松,吴军,易绍萱,贺伟峰,陈希炜,解志杰,彭双发,代飞.CD4^+CD25^+T_(reg)细胞调节T细胞的作用机制分析[J].第三军医大学学报,2003,25(21):1881-1884. 被引量:18
  • 7Green E A,Choi Y,Flavell R A.Pancreatic lymph node-derived CD4^+ CD25^ + Treg cells:highly potent regulators of diabetes that require TRANCE-RANK signals[J].Immunity,2002; 16(2):183-191.
  • 8Gregori S,Giarratana N,Smiroldo S et al.A lalpha,25-dihydroxyvitaminD(3) analog enhances regulatory T cells and arrests autoimmune diabetes In NOD mice[J].Diabetes,2002; 51(5):1367-1374.

二级参考文献13

  • 1Shevach E M. Regulatory T cells in autoimmunity[J]. Ann Rev Immunol,2000, 8(3) :423 - 449.
  • 2Suri-Payer E, Amar A Z, Thornton A, et al. CD4+ CD25+ T cells inhibit both the induction and effector function of autoreactive T cells and represent a unique lineage of immunoregulatory cells[J]. J Immunol, 1998, 160(3) :1212- 1218.
  • 3Masaki H, Cherry I K, Masanori N, et al. IL-10 is required for regulatory T cells to mediate tolerance to alloantigen in vivo [J]. J Immunol, 2001,166(9) :3789 - 3796.
  • 4Yamagiwa S, Gray J, Hashimoto S, et al. A role for TGF-β in the genera-tion and expansion of CD4+ CD25+ regulatory T cells from human peripheral blood[J]. J Immunol, 2001, 166(12) :7282 - 7289.
  • 5Sakaguchi S, Sakaguchi N, Asano M, et al. Immunological self-tolerance maintained by activated T cells expressing IL-2 receptor a chain. Breakdown of a single mechanism of self-tolerance causes various autoinr, nune diseases[J]. J Immnunol, 1995, 155(9): 1151-1164.
  • 6Akira Suto, Hiroshi Nakajima, Kei Ikeda, et al. CD4+ CIY25+ T-cell development is regulated by at least 2 distinct mechanisms [J]. Blood, 2002,99(7) : 555 - 560.
  • 7Takahashi T, Kuniyasu Y, Toda M, et al. Inr, ntmologic sel ftolerance maintained by CD25+ CD4+ naturally anergic and suppressive T cells: induction of autoinnntme disease by breaking their anergic/suppressive state[J]. Int Immunol, 1998, 10(9):1969-1980.
  • 8Read S, Malmstrom Y, Powrie F. Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25+ CD4+ regulatory cells that control intestinal inflammation[J]. J Exp Med, 2000, 192( 1 ):295 - 302.
  • 9Sakaguchi S. Regulatory T cells mediating compromises between host and parasite[J]. Nat Immnunol, 2003, 4( 1 ) : 10 - 11.
  • 10郑峻松,吴军,马兵,罗高兴,易绍萱,贺伟峰,陈希炜.CTLA4Ig基因转染猪皮异种移植的体外实验研究[J].第三军医大学学报,2001,23(7):762-764. 被引量:13

共引文献20

同被引文献84

引证文献7

二级引证文献21

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部