摘要
目的 建立螺旋藻多糖（APSP）脂质体包封率的测定方法。方法 用阴离子交换树脂分离脂质体和游离的APSP，采用分光光度法测定脂质体中APSP的含量，并计算包封率。结果 阴离子交换树脂对溶液中游离APSP吸附后的洗脱率达到95．4％，含量测定方法的线性范围为0～5．714μg／mL（r=0．9997），日内和日间精密度的RSD均小于2％（n=5），方法回收率为98．8％（n=5），APSP脂质体的平均包封率为23．9％。结论 此法简便、可行。
Purpose To prepare acidic polysaccharide of Spirulina plantensis （APSP） liposomes and to develop a method for determining its entrapment rate. Methods A column of DEAE ion exchange resin was used to separate the free APSP from the mixture of APSP liposomes preparation. The concentration of APSP in solution and the entrapment rate were determined by spectrophotometric method. Results The results indicated more than 95.4% of the free APSP in the liposomes preparation was adsorbed and later recovered in the eluent. The calibration curve for APSP by spectrophotometer at 482 nm was linear （ r = 0. 9997） when the concentration of APSP was in the range of 0-5.714 μg/mL. The precision tests on inter day and intra day determinations were all less than 2 % （ n = 5）. The recoveries of the method were 98.8 % （ n = 5）. The average entrapment rate of APSP in liposomes preparation was 23.9 %. Conclusion The described method is a simple and feasible one.
出处
《中国生化药物杂志》
CAS
CSCD
2006年第4期225-227,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
螺旋藻多糖
阴离子交换树脂
分光光度法
脂质体
包封率
acidic polysaccharide of Spirulina plantensis
DEAE ion enchange column
spectrophotometry
liposomes
entrapment rate
