摘要
目的探讨清开灵注射液对全氟异丁烯(PFIB)吸入性急性肺水肿的治疗作用。方法小鼠以全身暴露动态吸入PFIB方式染毒,剂量为130 mg/m3染毒5 m in。在小鼠PFIB染毒后1 h腹腔注射清开灵注射液,剂量分别为1.75、3.50、7.00、14.00 m l/kg。以染毒小鼠存活率、肺系数、支气管肺泡灌洗液(BALF)中蛋白含量及弹性蛋白酶活力、组织病理学检查等为观测指标,观察清开灵注射液抗PFIB吸入性急性肺水肿作用的量效关系。结果清开灵注射液可显著降低PFIB染毒小鼠的湿(干)肺体比、肺含水量及肺湿/干比,明显降低BALF中的蛋白含量,显著提高PFIB染毒小鼠的存活率,改善染毒小鼠的肺组织病变,且量效关系良好。结论清开灵注射液对PFIB所致肺水肿与炎性渗出具有肯定的抑制作用,并能有效对抗PFIB所致动物死亡。
Objective To investigate the therapeutic effect of Qingkailing injection on experimental acute pulmonary edema induced by perfluoroisobutylene （PFIB） inhalation. Methods Mice were intoxicated by flow-past whole-body exposure to PFIB at the concentration of 130 mg/m^3 for 5 minutes. One hour after the exposure, they were administrated Qingkailing injection intraperitoneally at the doses of 1.75, 3. 50, 7.00 and 14. 00 ml/kg respectively. Indexed by mice survival rate, lung coefficients, protein content and elastase activity in bronchoalveolar lavage fluid （ BALF）, as well as histopathologic alterations post PFIB exposure, the dose-response relationship of Qingkailing injection against acute pulmonary edema induced by PFIB inhalation was observed. Results Qingkailing injection could significantly decrease the wet （dry） lung-to-body weight ratio, water content in lung, lung wet-to-dry weight ratio as well as protein content in BALF in the PFIB-exposed mice in a well dose-depen-dent manner, which were well supported by the dramatically increased survival rate and ameliorated histopathologic alterations in the lung. Conclusion Qingkailing injection has the ascertained inhibitive effect on PFIB-induced pulmonary edema and inflammatory exudation and can effectively fight against PFIB-induced animal death.
出处
《中国职业医学》
CAS
北大核心
2006年第4期241-244,共4页
China Occupational Medicine
基金
国家"十五"863计划重大专项资助项目(2003AA2082034)
国家自然科学基金资助项目(30300280)
浙江省科技厅联合项目
关键词
全氟异丁烯
清开灵注射液
急性
水肿
肺
Periluoroisobutylene （PFIB）
Qingkailing injection
Acute
Pulmonary edema
