摘要
用分光光度法测定大鼠红细胞溶血程度,用丙二醛(MDA)试剂盒测定MDA。结果显示,通过腹膜注射菜籽肽,可以使小鼠血清中MDA含量显著减少。高剂量组[100 mg/(kg.d)]与对照组相比,MDA的降低达到极显著水平(P<0.01)。离体实验结果显示,菜籽肽粗品及各级分对体外温育和H2O2诱导的小鼠肝组织匀浆MDA生成有较强的抑制作用,其中粗品RSP-R和级分RSP-1的作用最好,且有量效关系,而对Fe2+诱导的MDA生成的抑制作用稍差。另外,RSP-R还可抑制大鼠红细胞氧化溶血程度。以上结果表明,菜籽肽在体内外均具有明显的抗氧化作用。
The effect of RSP on hemolysis of mice red blood cell was observed in the spectrophotometric method, while the reagent kits were used to analyse the content of malondialdehyde （ MDA ）. The results showed that MDA levels in serum in the RSP group were obviously lower than that of the control after injecting RSP for 12 d, there was a significant difference（ P 〈 0. 01 ） between the control group and the intra - peritoneal injected group （with RSP 100 mg/（ kg · d） 12 d） in MDA value in mice serum. In vitro, as for malondialdehyde（MDA） formation and H2O2 - induced of mice liver ,RSP - R and RSP - 1 had better inhibiting effects than RSP -2 and RSP - 3 , exhibiting dosage -depended, but little inhibiting effect on MDA induced by Fe^2 ＋. RSP -R can also inhibiting the hemolysis of mice red blood cell as well. All the results above show that RSP has antioxidation effect in vitro and in vivo.
出处
《中国油脂》
CAS
CSCD
北大核心
2006年第8期48-50,共3页
China Oils and Fats
基金
天津大学青年教师基金(5110107)
国家"十五"科技重点攻关计划(2001BA501A05)
关键词
菜籽清蛋白
菜籽肽
抗氧化
体内
体外
rapeseed albumin
rapeseed peptide （RSP）
antioxidation
in vivo
in vitro
