摘要
The strain Lv（1- z） isolated from the Henan bauxite was characterized by morphological observation, biochemical and physiological identification, and 16S rDNA sequence analysis. The influences of temperature, initial pH value, the volume of medium, shaking speed and illite concentration on the desilicating ability of the strain Lv（1- z） were investigated. The results show that the bacterium is a Gram-negative rod-shaped bacterium with oval endspores and thick capsule, but without flagellum. The biochemical and physiological tests indicate that the strain Lv（1- z） is similar to Bacillus rnucilaginosus. In GenBank the 16S rDNA sequence similarity of the strain Lv（1- z） and the B. rnucilaginosus YNUCC0001（AY571332） is more than 99%. Based on the above results, the strain Lv（1- z） is identified as B. rnucilaginosus. The optimum conditions for the strain L（1- z） to remove silicon from illite are as follows., temperature is 30℃ ;initial pH value is 7.5; medium volume in 200 mL bottle is 60 mL; shaking speed of rotary shaker is 220 r/m ; illite concentration is 1%.
The strain Lv_ 1-2 isolated from the Henan bauxite was characterized by morphological observation, biochemical and physiological identification, and 16S rDNA sequence analysis. The influences of temperature, initial pH value, the volume of medium, shaking speed and illite concentration on the desilicating ability of the strain Lv_ 1-2 were investigated. The results show that the bacterium is a Gram-negative rod-shaped bacterium with oval endspores and thick capsule, but without flagellum. The biochemical and physiological tests indicate that the strain Lv_ 1-2 is similar to Bacillus mucilaginosus. In GenBank the 16S rDNA sequence similarity of the strain Lv_ 1-2 and the B.mucilaginosus YNUCC0001(AY571332) is more than 99%. Based on the above results, the strain Lv_ 1-2 is identified as B.mucilaginosus. The optimum conditions for the strain Lv_ 1-2 to remove silicon from illite are as follows: temperature is 30 ℃;initial pH value is 7.5; medium volume in 200 mL bottle is 60 mL; shaking speed of rotary shaker is 220 r/m ; illite concentration is 1%.
基金
Project (50321402) supported by the National Natural Science Foundation of China
project (2004CB619204) supportedby the National Basic Research Program
