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毒害艾美球虫二次感染雏鸡外周血液免疫功能的变化 被引量:4

Changes of immune function of peripheral blood of chickens secondarily-infected with Eimeria necatrix
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摘要 将180只14日龄雏鸡随机分为毒害艾美球虫(Eimeria necatrix)初次感染组、二次感染组和对照组,应用免疫SPA菌体花环、间接ELISA及细胞培养技术和四甲基偶氮唑盐(MTT)测定法对相关免疫学指标进行了检测,以研究毒害艾美球虫二次感染对雏鸡外周血液免疫功能变化的影响。结果显示,毒害艾美球虫二次感染雏鸡外周血液T、B淋巴细胞数量及其对ConA或PMA的增殖反应和血清IgG、IgM、IgA免疫球蛋白含量均不同程度地高于未感染毒害艾美球虫的对照组雏鸡。证实毒害艾美球虫二次感染雏鸡外周血液的细胞免疫和体液免疫功能均明显提高。 One hundred and eighty 14-days-old chickens were divided randomly into coccidia primary infection group (I1), coccidia secondary infection group (I2), and control group(C). Immune indexes were examined by using immune staphytococcal protein A (SPA) rosette test, indirect enzyme-linked immunosorbent assay(IELISA),cellular culture technique and MTT method. The number and proliferative responses of T and B lymphocytes to ConA or phorbol myristate acetate (PMA), contents of IgG, IgM and IgA in peripheral blood of 14-days-old chickens secondarily infected with E. necatrix were elevated different extent compared with that of the control. The cellular and fluid immune function of peripheral blood of chickens secondarily-infected with E. necatrix were significantly increased.
出处 《中国兽医科学》 CAS CSCD 北大核心 2006年第8期643-646,共4页 Chinese Veterinary Science
基金 黑龙江省自然科学基金项目(C0206)
关键词 毒害艾美球虫 二次感染 雏鸡 外周血液 淋巴细胞数量和功能 免疫球蛋白 Eimeria necatrix secondary infection chicken peripheral blood number and function of T and B cells immunoglobulin
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参考文献9

  • 1Yun C H,Lillehoj H S,Lillehoj E P. Intestinal immune responses to coccidiosis[J]. Dev Comp Immunol, 2000,24 (2-3) : 303-324.
  • 2Williams R B. A compartmentalized model for the estimation of the cost of coccidiosis to the world's chicken production industry[J]. Int J Parasitol, 1999,29(8) : 1209-1229.
  • 3林青,于三科,张彦明,张志敏.柔嫩艾美耳球虫杨凌株对几种抗球虫药的抗药性研究[J].中国农学通报,2005,21(3):45-47. 被引量:11
  • 4吴绍强,蒋金书,刘群,朱引洁.鸡球虫疫苗研究进展[J].畜牧兽医学报,2005,36(1):1-5. 被引量:42
  • 5Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays[J].J Immunol Methods ,1983,65(1-2) :55-63.
  • 6夏生林,宋宏,陈成章,余红娥.间接ELISA法检测血清隐孢子虫抗体IgG及IgM的实验研究[J].中国人兽共患病杂志,2005,21(3):227-229. 被引量:4
  • 7Hein H. Pathogenic effects of Eimeria necatrix in young chickens[J]. Exp Parasitol, 1971,30(3):321-330.
  • 8Beattie S E,Barta J R,Fernando M A. Involvement of CD8^+ and CD3^ + lymphocytes in the transport of Eimeria necatrix sporozoites within the intestinal mucosa of chickens[J]. Parasitol Res ,2001,87(5):405-408.
  • 9Lillehoj H S,Min W,Dalloul R A. Recent progress on the cytokine regulation of intestinal immune responses to Eimeria[J]. Poult Sci ,2004,83(4) :611-623.

二级参考文献53

  • 1孔繁瑶,宁长申,殷佩云.15株柔嫩艾美耳球虫Eimeria tenella对五种抗球虫药的抗药性调查研究[J].北京农业大学学报,1994,20(3):302-308. 被引量:106
  • 2黄兵,史天卫,吴薛忠,赵其平,陈兆国.巨型艾美耳球虫纯种鉴定和致病性研究[J].中国兽医寄生虫病,1995,3(4):12-14. 被引量:19
  • 3蒋建林.[D].北京:中国农业大学,1999.
  • 4吴绍强 蒋金书 刘群 等.柔嫩艾美耳球虫BJ株真核融合表达载体的构建及其免疫保护效果研究[A]..中国畜牧兽医学会2003年年会论文集[C].北京,2003.21-24.
  • 5Girard F, Pery P, Naciri M, et al. Adjuvant effect of cholera toxin on systemic and mucosal immune responses in chickens infected with E. tenella or given recombinant parasitic antigen per os [J]. Vaccine, 1999, 17 :1516-1524.
  • 6Min W,Lillehoj H S,Burnside J,et al. Adjuvant effects of IL- 1β, IL-2, IL-8, IL-15, IFN-α, IFN-γ, TGF-β4 and lymphotaetin on DNA vaccination against Eimeria acervulina [J]. Vaccine, 2002,20 : 267 - 274.
  • 7Tomley F M,Billington K J,Bumstead J M,et al. EtMIC4:a microneme protein from Eimeria tenella that contains tandem arrays of epidermal growth factor-like repeats and thrombospondin type-I repeats[J]. Int JParasitol, 2001,31 : 1303 - 1310.
  • 8Chapman H D, Cherry T E,Danforth H D,et al. Sustainable coccidiosis contorl in poultry production: the role of live vaccines [J]. Int J Parasitol, 2002,32 : 617 - 629.
  • 9Wallach M G, Mencher D, Yarus S, et al. Eimeria maxima:identification of gametocyte protein antigens [J]. Exp Parasitol, 1989,68 : 49 - 56.
  • 10Wallach M, Halabi A, Pillemer G,et al. Maternal immunization with gametocytes antigens as a means of providing protective immunity against Eimeria maxima in chickens[J]. Infect Immun, 1992,60(5) :2036-2039.

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