摘要
采用两种处理方法对家兔进行了超数排卵研究。结果表明:PMSG-HCG法处理的母兔卵巢上的平均排卵点数极显著(P<0.01)高于PMSG法,而PMSG-HCG法的平均囊肿卵泡数与平均卵泡数则显著(P<0.05)低于PMSG法;两种方法间的平均卵泡发育数则差异不显著(P>0.05)。说明PMSG-HCG法的超排效果明显优于PMSG法。试验还表明,母兔交配后28h采胚所获取的胚胎大多处于二细胞期,到交配后32h,胚胎多处于四细胞期。采用PMSG-HCG法进行超排处理,对家兔的胚胎发育无影响。
Two methods were applied to research the superovulation of Rabbits. The results showed that the average number of the ovulation points on the ovaries of the rabbits using PMSG-HCG method was extremely different (P〈0.01) from those of PMSG method, and the average number of the cystic follicles and follicles of PMSG-HCG method was significant difference (P〈0.05) from that of PMSG method. The average number of the developed follieles of the two methods was not significant difference (P〉0.05). The superovulated effect of PMSG-HCG method was significantly better than that of PMSG method. The results also showed that most of the gained .embryos were in 2-cell stage during 28 hours after the rabbits accepted mating, and in 4-cell stage during 32 hours after the rabbits accepted mating. PMSG-HCG method didn't influence upon the development of the rabbits" embryos when it was applied to study on their superovulation effeet.
出处
《中国农学通报》
CSCD
2006年第8期44-46,共3页
Chinese Agricultural Science Bulletin
基金
河南省重大科技攻关项目(0422011200)资助。
关键词
家兔
超数排卵
胚胎发育
Rabbit, Superovulation, Embryo development