摘要
目的优选虎杖的白藜芦醇最佳提取工艺。方法①采用正交实验法,以白藜芦醇提取量为指标,高效液相色谱法(HPLC)测含量,优选提取工艺条件。②以大孔树脂富集及色谱法分离、纯化白藜芦醇。结果乙醇提取最佳工艺为10倍量的90%乙醇回流提取2次,1 h/次。经AB-8树脂吸附富集,硅胶柱色谱纯化,可获得高纯度的白藜芦醇产品。结论该工艺可用于指导生产。
Objective To study the optimum extraction process of resveratrol in Polygonum cuspidatum, Methods ①The optimum extraction process was investigated by orthogonal design and the index of the method was the contents of resveratrol, ②The technology of macroporous adsorptive resin and chromatography was used in the separation and purification of resveratrol by HPLC, Results The optimum condition of ethanol extraction ：10 times amount of 90% ethanol and extracted 2 times, 1 hour one time. The purity of the product was gained by the technology of resin AB-8 adsorption and silica column chromatography purification, Conclusion The optimum extraction process can direct production.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第8期1424-1425,共2页
Lishizhen Medicine and Materia Medica Research
基金
湖北省科技攻关资助项目(No.2005AA301C32)
关键词
虎杖
白藜芦醇
大孔树脂
硅胶柱层析
Polygonum cuspidatum
Resveratrol
Macroporous adsorptive resin
Slica column chromatography
