摘要
目的测定南瓜多糖的单糖组成及摩尔比。方法以1-苯基-3-甲基-5-吡唑啉酮(PMP)为单糖的柱前衍生化试剂,用毛细管区带电泳(CZE)法测定南瓜多糖的单糖组成。电泳缓冲液为pH10.8的150mmol·L-1硼砂水溶液;熔融石英毛细管柱(58.5cm×75μm)的有效长度为48.5cm;分离电压10kV;柱温25℃;3.45kPa压力进样,进样时间5s;二极管阵列检测器检测。结果各单糖的检测限均低于10μg·mL-1。南瓜多糖由木糖、阿拉伯糖、葡萄糖、鼠李糖、半乳糖、葡萄糖醛酸组成,其摩尔比为1.33∶3.12∶7.33∶1.0∶3.67∶6.06。结论该方法具有简单、快速、高效等优点,可用于南瓜多糖的单糖组成测定及质量控制。
OBJECTIVE To investigate monosaccharide composition and the molar ratio of pumpkin polysaccharides. METHODS The monosacoharides as 1-phenyl-3-methyl-5-pyrazolone （PMP） derivatives were separated by capillary zone electrophoresis （CZE）. The best condition was achieved with a fused silica capillary tube （58.5 ×75μm, effective length of 48.5cm）, 150mmmol·L^-1 sodium tetraborate buffer （ pH 10.8）, a constant voltage of 10kV at 25℃ and 0.5psi pressure injection for 5 second. RESULTS The lowest detection limits of the monosaccharides were blow 10μg·mL^-1. Pumpkin polysaccharides consisted of xylose, arabonose, glucose, rhamnose, galactose, glucuronic acid with the molar ratio of 1.33： 3.12： 7.33： 1.00： 3.67： 6.06. CONCLUSION This method is simple, rapid and sensitive. The method can be applied for the analysis of quality control and monosaccharide composition in pumpkin polysaccharides.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2006年第4期323-326,共4页
Chinese Journal of Modern Applied Pharmacy
关键词
南瓜多糖
衍生化
单糖组成
毛细管电泳
pumpkin polysaccharides
derivatization
monosaceharide composition
capillary zone electrophoresis
