摘要
目的:探讨高脂诱导肥胖模型大鼠血清瘦素、肿瘤坏死因子-α(TNFα-)、白细胞介素6(IL-6)的水平及其在胰岛素抵抗中的作用。方法:大鼠高脂饮食8周后,以体重比对照组重30 g为标准,分出肥胖大鼠,继续饲养4周,空腹麻醉下,心脏取血,分离血清。葡萄糖氧化酶法测血清血糖;放射免疫法(RIA)测血清瘦素、胰岛素;酶联免疫法(ELISA)测血清TNFα-、IL-6水平。以空腹胰岛素/血糖(Insulin/Glu-cose,I/G)比值作为胰岛素敏感指标。结果:与对照组相比,肥胖大鼠空腹胰岛素、I/G比值,瘦素、IL-6水平显著高于对照组(P均<0.05),血清TNFα-水平无差别;肥胖大鼠I/G比值与血清瘦素、IL-6均密切相关(r=0.772,P=0.036;r=0.898,P=0.015),与TNFα-不相关(r=0.341,P=0.254)。结论:肥胖大鼠血清瘦素、IL-6显著增高,参与了肥胖相关的胰岛素抵抗,血清TNFα-未增高,与肥胖相关的胰岛素抵抗无关。
Objective： To evaluate the role of serum leptin, TNF-α and IL-6 on insulin resistance in high-fat diet induced obese rats. Methods： After fed with high-fat diet for 8 weeks, the obese rats who were 30g heavier than controls were separated and fed for another 4 weeks. The blood samples were dmwed by heart puncture. The serum levels of glucose were measured by glucose oxide-peroxidase method, the levels of leptin and insulin by RIA, and the levels of TNF-α, IL-6 by ELISA. The ratio of insulin versus glucose（I/G） was used as an indicator for insulin resistance. Results： Compared with the controls, the serum levels of leptin, IL-6 and I/G were higher in the obese rats, but the levels of TNF-α were not different between two groups; The levels of leptin and IL-6, but not TNF-α , were closely related to the parameters evaluating insulin resistance in obese rats. Conclusions： The serum levels of leptin and IL-6 are increased in high-fat diet induced obese rats and contribute to insulin resistance. The levels of TNF-α in obese rats are neither statistically increased nor related to insulin resistance.
出处
《山东大学学报（医学版）》
CAS
北大核心
2006年第8期815-818,共4页
Journal of Shandong University:Health Sciences
