摘要
为了筛选出能综合双亲优势的杂种细胞，为废水资源化生产ＳＣＰ提供更为理想的菌株来源，将原核球形红假单胞菌细胞（ＮｔｒＳｍｓ）与真核酿酒酵母细胞（ＮｔｓＳｍｒ）的原生质体融合，构建成跨界杂种细胞（ＮｔｒＳｍｒ）．从中筛选出Ｆ１３，Ｆ１５，Ｆ２０三株高絮凝性融合细胞，在起始ＢＯＤ５浓度为１９１—６９０ｍｇ／Ｌ．ｐＨ７．０３０℃的味精废水中振荡反应７—８ｈ，测得融合细胞和双亲菌株降解利用有机污染物的系列反应动力学参数μｍａｘ，ｑｍａｘ，Ｋａμ，ＫｓｑＹ和Ｋｄ．发现融合细胞降解废水的性能具有优势，利于废水资源化处理．
he protoplasts of Rhodopseudomonas sphaerndes(eukaryote,NtrSms)and Saccharomyces cerevisiae (prokaryote, NtsSmr)were induced with PEG(MW= 6000)to fuse for the construction of the fusant cell (NtrSmr).The fusants F13,F15 and F20 screened from the'fusion production with high flocculation were used to test the kinetics parameters for degradation of MSG wastewater in the shaking reaction for 7-8 h,while the initial BOD5 concentration of the wastewater were from 191 to 690 mg/L.The values of maximum specific growth rate umax. and its half velocity constant Ksu,the maximum specific degradation rate qmaxand its half velocity constant Ksq the true growth yield coefficient Y and the endogenous decay rate coefficient Kd were measured in the reaction.The results of this research suggest that the fusants F13,F15 and F20 have better ability for degradation.of the organic waste in MSG wastewater.
出处
《环境科学》
CAS
CSSCI
CSCD
北大核心
1996年第3期5-7,共3页
Environmental Science
基金
国家自然科学基金
关键词
酿酒酵母
原生质体融合
味精废水
光合细菌
Rh.sphaeroides,S.cerevisiae fusant,monosodium glutamate wastewater,biodegradation kinetic.
