摘要
Porous chitosan microspheres with diameters ranging from 180μm to 280 μm were successfully prepared, using an anti-phase suspension method combined with temperature controlled freeze-extraction. The mean pore diameter could be regulated from 5 μm to 60μm by varying the freezing temperature through the cooling rate. Results with in vitro chondrocyte cultures showed that cells could attach, proliferate and spread on these porous microspheres as well as inside the microcarriers. The materials and cell cocultures were characterized using both optical and scanning electron microscopy. These results show that the porous chitosan microspheres are promising candidates for tissue culture for use as an injectable tissue engineering scaffold.
Porous chitosan microspheres with diameters ranging from 180μm to 280 μm were successfully prepared, using an anti-phase suspension method combined with temperature controlled freeze-extraction. The mean pore diameter could be regulated from 5 μm to 60μm by varying the freezing temperature through the cooling rate. Results with in vitro chondrocyte cultures showed that cells could attach, proliferate and spread on these porous microspheres as well as inside the microcarriers. The materials and cell cocultures were characterized using both optical and scanning electron microscopy. These results show that the porous chitosan microspheres are promising candidates for tissue culture for use as an injectable tissue engineering scaffold.
基金
Supported by the National Key Basic Research and Development (973) Program of China (No. 2005CB623905) and the Tsinghua-Yue-Yuen Medical Science Fund
