摘要
目的观察大鼠肢体缺血/再灌注(LIR)后心肌组织的损伤性变化并探讨氧化应激在其发生机制中的作用。方法用健康雄性Wistar大鼠制备LIR动物模型,采用生物化学方法及比色法测定心肌组织丙二醛(MDA)、髓过氧化物酶(MPO)、ATP、乳酸(LD)含量及ATP酶(ATPase)活性;测定血浆肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)水平,光镜观察心肌组织的形态学变化。结果与对照组比较,再灌注后心肌组织ATP储备减少,LD含量增加,各型ATPase活性下降,血浆CK、CK-MB及心肌组织MDA、MPO含量均增高(P<0·05或P<0·01)。镜下可见再灌注后心肌组织炎性细胞浸润,部分心肌横纹不清晰,少数心肌细胞变性。结论LIR可对心肌组织造成一定损伤,这与体内的氧化应激状态有密切关系。
Objective To observe the injury of myocardium and to investigate the role of oxidative stress after limbs ischemia reperfusion in rats. Methyls 20 male healthy Wistar rats were randomly divided into 2 groups： control group（C） and ischemia-reperfusion group （IR）. The biochemical methods were used to measure the contents of rnalondialdehyde （MDA）, myeloperoxidase （MPO）, ATP, lactic acid （LD） and ATPase of myocardium. The levels of creatine kinase （CK） and creatine kinase MB isoenzyme （CK-MB） in plasma were also observed. The morphologic changes of myocardium were observed with microscope. Results It was found that after rats＇ limbs suffering from ischemia-reperfusion, the level of ATP decreased, the activities of three kinds of ATPase （Na^＋ -K^＋-ATPase, Ca^2＋ ATPase and Mg^2＋ ATPase） decreased in different degree （P〈0. 01 or P〈0. 05）. On the contrary, the contents of CK, CK-MB in plasma and LD, MDA, MPO of myocardium increased obviously. The differentiations were all significant. Under microscope, inflammatory cellular infiltration was obvious, cross striation in some area was not clear and some myocardial cells had degenerated in IR group. Conclusion The myocardium injury happened after limbs ischemia reperfusion in rats. The damage may be related to oxidative stress.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2006年第8期802-803,共2页
Medical Journal of Chinese People's Liberation Army
关键词
再灌注损伤
心肌
损伤
reperfusion injury
myocardium
injury
