摘要
目的研究凋亡抑制基因Survivin和抑癌基因PTEN在膀胱移行细胞癌(BTCC)中的表达及其与肿瘤分级、分期的关系。方法应用免疫组织化学方法,检测60例膀胱移行细胞癌和10例正常膀胱黏膜组织中Survivin蛋白和PTEN的表达。结果60例BTCC中Survivin蛋白的表达率为76.67%,10例正常膀胱组织中未见Survivin蛋白表达,Survivin蛋白表达随BTCC分级、分期的增高而增高。Survivin蛋白在各级肿瘤中表达Ⅰ级42.86%,Ⅱ级79.17%,Ⅲ级95.45%;浅表性癌表达率55.56%,浸润性癌表达率为85.71%,初发63.64%,复发92.59%,单发61.54%,多发88.24%;初发与复发,单发与多发之间表达具有统计学意义(P<0.05)。PTEN蛋白在60例膀胱移行细胞癌组织中阳性表达率为31.67%,PTEN阳性表达率随肿瘤细胞病理分级、临床分期程度的增高呈下降趋势。10例正常膀胱组织中PTEN蛋白均呈阳性反应。结论Survivin蛋白和PTEN的表达与BTCC临床病理特征和生物学行为有密切关系,联合检测Survivin和PTEN,对诊断BTCC、判断其恶性程度及预后均有一定参考价值。
Objective To study the expression of survivin, PTEN in BTCC and its relationship between tissue grade and pathology stage. Methods The immunohistological staining was used to evaluated the expression of survivin protein and PTEN in 60 cases of transitional cell carcinoma of urinary bladder and 10 normal bladder mucosa. Results The positive rate of survivin protein in BTCC was 76.67%, expression of survivin protein in 10 normal mucosa was negative; In BTCC, grade Ⅰ 42.86%, Ⅱ 79.17%, Ⅲ 95.45% ; in stage of T1 ,T2-3 are 55.56% ,85.71% respectively; and in primary,recurrent cases are 63.64%, 92.59% （P 〈 0.05） respectively. The positive rate of PTEN was 31.67%, the higher the grade and the clinical stage of tumors, the lower the expression of PTEN, 10 normal bladder mucosa was positive. Conclusion The positive correlation was found between the abnormal expression of survivin protein, PTEN and the behavior in biology of the transitional cell carcinoma of the bladder. The coexpression of survivin and PTEN have the synergism in diagnosis, malignant degree and prognosis in BTCC.
出处
《实用肿瘤学杂志》
CAS
2006年第4期270-273,共4页
Practical Oncology Journal
