摘要
采用CaCo2细胞单层肠上皮系统感染模型,将5×10-5mmol/L,1×10-4mmol/L和2×10-4mmol/L 3种不同浓度的黄芪多糖及不含黄芪多糖DMEM细胞培养液分别加入已平铺于培养板的CaCo2细胞单层系统中与定量的大肠埃希菌混合培养,观察侵入细胞的大肠埃希菌量。结果黄芪多糖浓度为5×10-5,1×10-4mmol/L和2×10-4mmol/L,处理的CaCo2细胞液的细菌培养数量分别为252.5个±54.5个,240.8个±44.2个和292.7个±71.3个,而对照组为598.8个±114.8个,显示黄芪多糖能显著减少大肠埃希菌进入上皮细胞的数量,增强肠黏膜的屏障功能。
The model of CaCo2 cells monolayer system has been generally accepted as a standard method for studying of the relationship between the epithelium and microbial invasion in vitro. In this study, the CaCo2 cells monolayer systems were co-cultured with 5× 10^-5mmol/L, 1 × 10^-4 mmol/L, and 2 × 10^-4mmol/L astragalus polysaccharides（APS） from huang qi for 2 hours, and with frank DMEM as control. Bacterial invasion was assessed by quantitating the clone counts of E. coli within the coultured CaCo2 cells. The results showed that APS significantly inhibited the invasion of E. coli to epithelial cells（P〈0.01）. The clone counts in c,ycultures with 5 × 10^-5 mmol/L, 1 × 10^-4mmol/L and 2× 10^-4mmol/L APS were 252.5 ±54. 5, 240. 8±44.2 and 292.7±71.3,respectively, compared with 598.8±114. 8 in DMEM control group. Conclusion. APS can protect the intestinal epithelium against E. coli invasion.
出处
《动物医学进展》
CSCD
2006年第8期85-87,共3页
Progress In Veterinary Medicine
基金
贵阳市科技局农业发展基金(2005筑科农字第4-10号)
贵州大学人才基金资助
关键词
黄芪多糖
CaCo2
细菌移位
astragalus polysaccharides CaCo2 bacterial translocation
