摘要
采用高效液相色谱法测定金银花及其制剂“利咽止咳冲剂”中绿原酸的含量。以ＫＹＷＧ－Ｃ＿（１８）为固定相，水－乙腈－冰醋酸（１１０：３：３）为流动相，检测波长为３２３ｎｍ，绿原酸在０．０１～０．１０ｍｇ／ｍｌ浓度范围与峰面积呈直线关系，回归方程为Ｙ＝一２．４６０５ｘ１０３＋５．６１３８×１０￣３Ｘ，ｒ＝０．９９９８。平均回收率为１００．８７％，ＲＳＤ为１．５５％（ｎ＝６）。本法简便，快速，结果可靠。
Chlorogenic acid in Flos lonicerae and“Li Yan Zhi Ke Chong Ji”was determinatedby high performance liquid chromatography.Chlorogenic acid was separated on KYWG-C18 col -umn and detected at 323nm,using water-acetonitrile-acetic acid(110:3:3)as mobile phase. There is a goOd linearity from 0.01 to 0.10 mg/ml of gatrodin(r=0.9998,Y=一2.4605x103 +5. 6138 x 105X). The.average recovery was 100.87%and the relative standard deviation was1. 55%(n=6).This method is simple,rapid and reliable.
出处
《中国实验方剂学杂志》
CAS
1996年第5期5-7,共3页
Chinese Journal of Experimental Traditional Medical Formulae
