摘要
目的探讨免疫调节性T细胞(Treg细胞)/CD4^+T细胞比值改变在动物急性感染后肠功能紊乱发病和预防机制中的作用。方法40只雌性Wistar大鼠均分为正常对照组、实验对照组、抗生素治疗组、微生态制剂治疗组和抗生素与微生态制剂联合治疗组。应用福氏痢疾杆菌灌胃造成肠道急性感染后,正常组和实验对照组每日0．9％氯化钠溶液1 ml灌胃,连续10 d；抗生素治疗组用庆大霉素8．32 mg·kg^(-1)·d^(-1)灌胃,连续3 d,后7 d 0．9％氯化钠溶液1 ml灌胃；微生态制剂治疗组用多维乳酸菌(美常安)52 mg·kg^(-1)·d^(-1)灌胃,连续7 d,后3 d 0．9％氯化钠溶液1 ml灌胃；联合治疗组前3 d每日应用庆大霉素,后7 d每日用多维乳酸菌灌胃。在感染成功后16～22 d,流式细胞仪分析外周血和肠系膜淋巴结中Treg细胞比例,并测定肠道感觉阈值和平滑肌张力积分,远端结肠行病理学检查。数据行t检验和方差分析。结果各组大鼠组织学均无明显变化；实验对照组大鼠肠道感觉阈值明显降低,平滑肌张力积分明显高于正常对照组(P＜0．05),外周血和肠系膜淋巴结Treg/CD4^+T细胞比值[(4．40±0．43)％,(2．47±1．36)％]较正常对照组明显降低[(5．15±0．47)％,(4．01±1．12)％,P＜0．05]；联合应用抗生素和微生态制剂治疗,可使比值上升至正常水平[(4．95±0．41)％,(3．22±1．86)％],肠道感觉阈值和平滑肌张力积分恢复正常。结论Treg/CD4^+T细胞改变在动物急性感染后肠功能紊乱发病机制中起重要作用,在急性肠道感染后,联合应用抗生素和微生态制剂治疗可使比值恢复正常,预防肠功能紊乱发生。
Objective To investigate the role of Treg/CD4^＋ T in the pathogenesis and prevention of bowel dysfunction after aeute enteritis in rats. Methods Studies were conducted in 40 female Wistar rats with weights of 170-180 g. The rats were divided into normal control, experimental control, antibiotics-treated, probiotics-treated and combined treated groups. The latter 4 groups were first infected with shigella flexneri by gavage to establish the model of acute infectious enteritis, then normal control and experimental control group were administered 1 ml saline once everyday for 10 days. Antibiotics- treated group was given gentamycin 1 ml （8.32 mg· kg ^-1 . d^-1 ） for 3 days, then was given saline 1 ml once everyday for 7 days. Probiotics-treated group was given medilac-S 1 ml （52 mg · kg^-1· d 6-1 ） by gavage for the first 7 days, and 1 ml saline for the latter 3 days. The combined treatment group was given gentamycin for the first 3 days and medilac-S for the latter 7 days using the same dose. All rats were given colon-rectal balloon distention 16 to 22 days after the occurrence of bowel inflammation. The contraction of enteric smooth muscle was measured in vitro, the proportion of Treg cells in blood and mesenteric lymph node were analyzed by flow cytometry, and the histological changes were also observed at the same time. Statistical analyses were performed by usingt test or one wayANOVA.Results No obvious changes of distal colon were histological observed in different groups. The perceptual threshold of rat gut decreased and the tension integral of enteric smooth muscle increased, and the ratio of Treg/CD4^＋ T in blood and mesenteric lymph node was significantly lower in experimental control group[-（4.40±0.43）% （2.47±1.36）%]thannormalcontrols[（5.15±0.47）% （4.01 ±1.12）%, P〈 0.05], while that of combined treatment group had no difference from normal controls [（4.95±0.41）% （3.22±1.86）%]. Conclusions The ratio ofTreg/CD4^＋ T may playanimportant role in the pathogenesis of post-infectious bowel dysfunction in animals. Treating acute enteritis with both antibiotics and probiotics can normalize the abnormalities, and thus prevent the occurrence of bowel dysfunction.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2006年第7期435-439,共5页
Chinese Journal of Digestion
