摘要
目的:分析幽门螺杆菌(HP)相关性慢性活动性胃炎患者胃窦黏膜组织内肿瘤坏死因子(TNF)的作用。方法:HP(+)慢性活动性胃炎患者19例(根据慢性活动性胃炎炎症活动度分级:Ⅰ级6例,Ⅱ级5例,Ⅲ级8例),HP(-)慢性活动性胃炎患者18例,正常胃黏膜11例,均经胃镜和病理组织学检查确诊。16例HP(+)慢性活动性胃炎患者均接受1周抗HP治疗。取胃窦部活检组织培养,用RT-PCR检测TNF-αmRNA表达水平;利用ELISA法检测培养上清液中TNF-α蛋白的水平。结果:HP(+)慢性活动性胃炎患者黏膜组织TNF-α蛋白分泌水平及组织内mRNA水平均高于HP(-)慢性活动性胃炎患者和正常组(P<0.05)。Ⅲ级HP(+)慢性活动性胃炎患者胃窦黏膜组织TNF-α蛋白分泌水平高于Ⅰ、Ⅱ级患者(P<0.05)。HP(+)慢性活动性胃炎患者经抗HP治疗后,胃窦黏膜组织中TNF-α分泌量降低(P<0.05)。结论:HP感染可明显诱导胃窦黏膜组织TNF-α表达;TNF-α在慢性活动性胃炎发生中起重要作用。
Aim To analyze the role of tumor necrosis factor （ TNF）-α in the pathogenesis of Helicobacter pylori （HP）-associated chronic gastritis. Methods： Nineteen patients with HP-associated chronic gastritis, eighteen patients with HP-negative chronic gastritis, and 11 healthy controls were subjected. Sixteen patients with HP-associated chronic gastritis accepted treatment against HP infection for a week. Antral biopsies were taken and cultured for 48 h, and TNF-α mRNA levels were assessed using RT-PCR assay and supernatants were collected to detect TNF-α using ELISA. Results： The level of TNF-α protein in the supernatants and the expression of TNF-α mRNA in antral biopsies were higher in patients with HP-positive chronic gastritis than those in HP-negative chronic gastritis and healthy controls （ P 〈 0.05 ）. TNF-α level was decreased in the supernatants from patients with HP-associated gastritis after the treatment against HP infection （P 〈 0.05 ）. Conclusion ： TNF-α is highly expressed in antral mucosa after HP infection, which may play an important role in the pathogenesis of chronic gastritis.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2006年第5期833-835,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金资助项目30571751
关键词
胃炎
慢性
幽门螺杆菌
肿瘤坏死因子-α
gastritis,chronic
helicobacter pylori
tumor necrosis factor-α
