摘要
目的:建立反相高效液相色谱法测定人血浆中兰索拉唑的浓度。方法:色谱柱为Agilent Eclipse XDB C_(18)(150 mm×4．6 mm,5μm),流动相为0．01 mol·L^(-1)磷酸二氢铵(内含1‰正辛胺)-乙腈(66:34),流速为1．0 ml·min^(-1),检测波长为285 nm,内标为奥美拉唑。结果:兰索拉唑的线性范围为0．01-2．00μg·ml^(-1),回归方程为Y=0．0019C+0．0022(r=0．999 4);批内RSD为2．95％～3．25％,批间RSD为1．09％-1．95％。结论:本方法操作简便,测定结果准确,检测限较低,重现性好,可用于兰索拉唑人体药物动力学研究。
Objective： To establish a RP-HPLC method for the determination the content of Lansoprazole in human plasma. Method： The Agilent Eclipse XDB C18 column （150 mm × 4.6 mm, 5 μm） was used with mobile phase of 0.01 mol·L^-1 ammonium hihydrogen phosphate-acetonitrile （66： 34） to which 1%o Octylamine was added. The flow rate was 1.0 ml·min^-1. The detection wavelength was 285 nm（lansoprazole） and 305nm （ omeprazole）. Internal standard was omeprazole. Result： The linear range of the calibration was （0.01-2.00）μg·ml^-1（ Y=0.0019C ＋0.002 2, r =0.999 4）. Within-day RSD was 2.95%-3.25%. Between-day RSD was 1.09%-1.95%. Conclusion： The method is simple, accurate, it can be used in the phamarmacokinetic study of lansoprazole.
出处
《中国药师》
CAS
2006年第9期823-825,共3页
China Pharmacist
