期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

宫颈癌患者血浆和组织中FHIT基因5′端CpG岛甲基化状态的研究 被引量：12

Methylation Status of the 5′ CpG Islands in FHIT Gene in the Plasma and Tissues of Cervical Cancer Patients
下载PDF
导出
摘要 为了检测宫颈癌患者血浆和组织中FHIT基因5′端CpG岛甲基化状态,以找到无创伤性诊断宫颈癌的新指标,选取151例宫颈癌患者的血浆和30例患者的宫颈癌组织为研究对象,用MSP的方法检测FHIT基因5′端CpG岛甲基化状态,并对MSP产物进行克隆和测序。结果在宫颈癌患者血浆和组织中,FHIT基因5′端CpG岛甲基化率为30.46%和53.33%,血浆和组织的总体符合率为80%。而对照中均未检测到甲基化状态。随着患者临床分期和组织学分级的增加,FHIT基因甲基化的检出率也在逐渐的增加。表明宫颈癌患者的血浆和肿瘤组织中FHIT基因5′端CpG岛甲基化的发生是高频事件,使用FHIT基因作为标记可以对宫颈癌患者进行无创伤诊断和预后的评估。 To evaluate the methylation status of the 5＇CpG islands in FHIT gene using plasma and tissue samples from cervical cancer patients and find a novel marker for non-invasive diagnosis of cervical cancer, methylation-specific PCR （MSP） was employed to examine CpG island methylation in FHITgene in 151 pretreatment plasma samples and 30 tumor tissue samples obtained from cervical cancer patients. MSP product was cloned and sequenced directly. CpG island methylation of FHIT was detected in 31.13% of the plasma samples, and in 53.33% of the tissue samples. The total concordant rate of methylation status between plasma and tissue samples in FHIT gene was 80.00%. We found a strong positive correlation between ,FHIT methylation in the plasma and the clinical stage and histological grade of the tumor. The data showed that CpG island methylation of the FHIT gene is prevalent in the plasma and tissue samples from cervical cancer patients. FHIT detection may be used as a non-invasive marker for diagnosis of cervical cancer and prognostic treatment evaluation.
作者 任晨春 苗绪红 杨斌 赵磊 孙蕊 宋文芹
机构地区 天津市中心妇产科医院遗传室 南开大学生命科学院
出处 《遗传》 CAS CSCD 北大核心 2006年第9期1061-1066,共6页 Hereditas（Beijing)
基金 天津市131人才基金资助~~
关键词 宫颈癌 甲基化状态 FHIT基因 血浆 组织 cervical cancer methylation status FHIT gene plasma tissue
分类号 R737.33 [医药卫生—肿瘤]
  • 相关文献

参考文献20

  • 1Ohta M,Inoue H,Cotticelli M G,Kastury K,Baffa R,Palazzo J,Siprashvili Z,Mori M,McCue P,Druck T,Croce C M,Huebner K.The FHIT gene,spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint,is abnormal in digestive tract cancers.Cell,1996,84(4):587～597.
  • 2Zochbauer-Muller S,Fong K M,Maitra A,Lam S,Geradts J,Ashfaq R,Virmani A K,Milchgrub S,Gazdar A F,Minna J D.5' CpG island methylation of the FHIT gene is correlated with loss of gene expression in lung and breast cancer.Cancer Res,2001,61(9):3581～3585.
  • 3Zou M,Shi Y,Farid N R,al-Sedairy S T,Paterson M C.FHIT gene abnormalities in both benign and malignant thyroid tumors.Eur J Cancer,1999,35(3):467～472.
  • 4史惠蓉,吴庆华,索振河,Jahn M Nesland.宫颈癌组织中FHIT基因5'端CpG岛甲基化及其与基因失活的关系[J].癌症,2005,24(1):7-11. 被引量：20
  • 5Tanaka H,Shimada Y,Harada H,Shinoda M,Hatooka S,Imamura M,Ishizaki K.Methylation of the 5'CpG island of the FHIT gene is closely associated with transcriptional inactivation in esophageal squamous cell carcinomas.Cancer Res,1998,58(15):3429～3434.
  • 6Kannan K,Munirajan A K,Bhuvarahamurthy V,Mohanprasad B K,Shankar P,Tsuchida N,Shanmugam G.FHIT gene mutations and single nucleotide polymorphism in Indian oral and cervical squamous cell carcinomas.Oral Oncol,2000,36(2):189～193.
  • 7Hendricks D T,Taylor R,Reed M,Birrer M J.FHIT gene expression in human ovarian,endometrial and cervical cancer cell lines.Cancer Res,1997,57(11):2112～2115.
  • 8Hayashi S,Tanimoto K,Hajiro-Nakanishi K,Tsuchiya E,Kurosumi M,Higashi Y,Imai K,Suga K,Nakachi K.Abnormal FHIT transcripts in human breast carcinomas:a clinicopathological and epidemiological analysis of 61 Japanese cases.Cancer Res,1997,57(10):1981～1985.
  • 9Druck T,Hadaczek P,Fu T B,Ohta M,Siprashvili Z,Baffa R,Negrini M,Kastury K,Veronese M L,Rosen D,Rothstein J,McCue P,Cotticelli M G,Inoue H,Croce C M,Huebner K.Structure and expression of the human FHIT gene in normal and tumor cells.Cancer Res,1997,57(3):504～512.
  • 10Panagopoulos I,Thelin S,Mertens F,Mitelman F,Aman P.Variable FHIT transcripts in non-neoplastic tissues.Genes chromosomes cancer,1997,19(4):215～219.

二级参考文献46

  • 1Ohta M, Inoue H, Cotticelli MG, et al. The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t (3 ;8) breakpoint, is abnormal in digestive tract cancers [J]. Cell, 1996, 84(4): 587-597.
  • 2Pekarsky Y, Zanesi N, Palamarchuk A, et al. FHIT: from gene discovery to cancer treatment and prevention [J]. Lancet Oncol, 2002, 3(12) :748-754.
  • 3Zochbauer-Muller S, Fong KM, Maitra A, et al. 5' CpG island methylation of the FHIT gene is correlated with loss of gene expression in lung and breast cancer [J]. Cancer Res, 2001, 61 (9) :3581-3585.
  • 4Maruyama R, Toyooka S, Toyooka KO, et al. Aberrant promoter methylation profile of prostate cancers and its relationship to clinicopathological features [J]. Clin Cancer Res, 2002, 8(2):514-519.
  • 5Noguchi T, Takeno S, Kimura Y, et al. FHIT expression and hypermethylation in esophageal squamo.s cell carcinoma [J]. Int J Mol Med, 2003, 11(4):441-447.
  • 6Maruyama R, Sugio K, Yoshino I, et al. Hypermethylation of FHIT as a prognostic marker in nonsmall cell lung carcinoma [J]. Cancer, 2004, 100(7) : 1472-1477.
  • 7Liu FS, Hsieh YT, Chen JT, et al. FHIT (fragile histidine triad) gene analysis in cervical intraepithelial neoplasia [J]. Gynecol Oncol, 2001, 82(2):283-290.
  • 8Chaudhuri AR, Khan IA, Prasad V, et al. The tumor suppressor protein Fhit. A novel interaction with tubulin [J]. J Biol Chem, 1999, 274(34):24378-24382.
  • 9Sard L, Accomero P, Tomielli S, et al. The tumor-suppressor gene FHIT is involved in the regulation of apoptosis and in cell cycle control [J]. Proc Natl Acad Sci USA, 1999, 96( 15 ) : 8489-8492.
  • 10Ji L, Fang B, Yen N, et al. Induction of apoptosis and inhibition of tumorigenicity and tumor growth by adenovirus vector-mediated fragile histidine triad (FHIT) gene overexpression [J]. Cancer Res, 1999, 59(14) :3333-3339.

共引文献26

同被引文献81

引证文献12

二级引证文献31

遗传
2006年 第9期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部