摘要
目的建立应用PCR技术进行RHCE基因分型的方法。方法应用序列特异性引物PCR技术(PCR-SSP)检测200例中国北方汉族、南方黎族个体的RHCE基因型,同时对5例亲子鉴定样品进行检测。结果2个民族个体RHCE基因分型结果与血清学分型结果完全一致;其中中国北方汉族RH基因型频率分布为RHCCEE1例,RHCCEe3例,RHCCee88例,RHCcEE4例,RHCcEe20例,RHCcee54例,RHccEE1例,RHccEe22例,RHccee7例;中国南方黎族RH基因型频率分布为RHCCEE2例,RHCCEe2例,RHCCee106例,RHCcEE7例,RHCcEe62例,RHCcee10例,RHccEE3例,RHccEe8例。亲子鉴定样品RHCE基因型检测结果与13个STR位点联合鉴定结论一致。结论PCR-SSP技术能准确判断中国北方汉族和南方黎族个体的RHCE基因型。
Objective To establish a method which processes RHCE genotyping with PCH. Methoas Using PCR-SSP to detect RHCE genotype in 200 cases of Han population in north of China and Li population in south of China and detecting 5 samples of parentage testing at the same time. Results The results of RHCE genotyping in individuals of two populations are completely accorded with the resuhs of serology typing. And the distribution of RH genotypic frequency in Han population in north China is： RHCCEE 1, RHCCEe 3, RHCCee 88, RHCcEE 4, RHCcEe 20, RHCcee 54, RHccEE 1, RHccEe 22, RHccee; The distribution of RH genotypic frequency in Li populatin in south China is RHCCEE 2, RHCCEe 2, RHCCee 106, RHCcEE 7, RHCcEe 62, RHCcee 10, RHccEE 3, RHccEe 8. The resuhs of RHCE genotype detecting of parentage testing samples are accorded with the results of associated identification of 13 STR loci. Conclusion PCR-SSP technology can exactly detect RHCE genotype.in individuals of Han population in north China and Li population in south China.
出处
《法医学杂志》
CAS
CSCD
2006年第4期271-274,共4页
Journal of Forensic Medicine
