摘要
研究了在有机溶剂中,Novozym435脂肪酶催化正丁醇和正己酸反应的动力学。结果表明,反应初速率V0与正丁醇和正己酸的摩尔浓度的比值(C0ROH/C0HA)有关。随着C0ROH/C0HA的增大V0先是增大,至C0ROH/C0HA近1时达最大值,之后V0减小。即两底物摩尔数接近相等时,在酶的活性中心部位两底物可充分反应,V0最大。另外,考察了不同醇类和不同有机溶剂对酶促反应的影响。随着醇碳链的增长,反应初速率增加。在疏水性强的烷烃中反应速率较大,在卤代烃及极性的MIBK等中速率较小。
The kinetics of the esterification reaction of hexanoic acid with butanol catalyzed by Novozym435 lipase wasstudied in organic solvents. The result shows that the intial rate V0 is related to the ratio of tool concentration C°ROH/C°HA, As C°ROH/C°HA increases, Vo increases and get the maximum value, and afterwards V0 decreases. As the alcohol chainlength increases, the initial rote also increases and reaches a ＂plateau＂ region. The magnitude order of the initial rate in different organic solvents is： hexane 〉 octane〉toluene 〉 cyclohexane 〉 benzene 〉 MIBK 〉 CC14 〉 chloroform. That is, the reaction is easier to take place in lipophilic solvents.
出处
《山东科学》
CAS
2006年第4期6-10,共5页
Shandong Science
基金
山东省自然科学基金资助(Y2003B01)
关键词
酯合成反应
脂肪酶催化
动力学
有机溶剂
esterification reaction
lipase catalysis
kinetics
organic solvent
