摘要
目的研究人肝星状细胞系（LX-2细胞）生长抑素受体（somatostatin receptor，SSTR）亚型的表达情况，探讨其临床意义。方法选择对数生长期的LX-2细胞，将细胞以8×10^6个／孔接种于预置入盖玻片的6孔板中，分为LX-2组、中药复方861组、奥曲肽（octreotide）处理组及SSTR亚型阳性细胞（PANC-1）对照组，每组重复2孔。应用免疫细胞化学染色方法，观察LX-2细胞SSTR2、SSTR5的表达；用RT-PCR方法观察SSTR亚型1～5的表达。结果免疫细胞化学染色结果发现，LX-2细胞SSTR亚型2、5表达阳性，阳性物质呈棕红色，主要位于细胞膜及细胞质，复方861处理组SSTR2、5表达增强，细胞着色较深；RT—PCR结果发现，LX-2细胞SSTR亚型1～5的表达均阳性，阳性表达的高低依次为SSTR1、SSTR4〉SSTR2、SSTR5〉SSTR3（P〈0．05）。结论LX-2表达5种SSTR亚型，肝星状细胞可能是SST及其类似物降低门静脉压力的作用靶点。奥曲肽可能通过SSTR2、SSTR5亚型调节肝星状细胞的舒张与收缩功能，从而影响肝内阻力及门静脉压力。
Objective To study the expressions of somatostatin receptor subtypes in human activated hepatic stellate cells （LX-2）. Its clinical significances was also surveyed. Methods To detect the expressions of SSTR2/5 of LX-2 which is in the logarithmic growth cycle using immunocytochemistry staining method： LX-2 were routinely cultured on small glass coverslip in six well cluster dishes at a density of 8 × 10g cells/well. The cells were divided into 5 subgroups： compound 861 0.5 g/L,compound 861 1.0 g/L,octreotide 1 mg/L and control group,PNAC-1 was a positive control cell line. Meantime,expressions of SSTR1 -5 mRNA were observed using RT-PCR method. Results The expressions of SSTR 2, SSTR5 in LX-2 were positive after immunocyto-chemistry staining. The mainly staining site is cellular membrane and cellular plasm. The strong expressions in compound 861-treated group were also observed. The five kinds of subtypes of SSTR in LX-2 were proven to be positive via the detection of RT-PCR. To compared the level of the subtypes, SSTR1,4〉SSTR2,5〉SSTR3 was observed. Conclusion LX-2 expresses five kinds of somatostatin receptors subtype. It is suggested that the activated hepatic stellate cells is target of SST and its analogs.
出处
《首都医科大学学报》
CAS
2006年第4期441-444,共4页
Journal of Capital Medical University
基金
北京市科技新星计划(954812500)资助项目
