摘要
目的：探讨人参总皂甙(TSPG)协同造血生长因子体外诱导CD34^+造血干/祖细胞(HSC/HPC)增殖的作用。方法：收集人脐血细胞,采用Stemsep TM干细胞分选系统分离纯化CD34^+HSC/HPC,经不同剂量TSPG加入不同造血生长因子组合进行培养,检测细胞总数、CD34^+细胞及集落形成细胞总数(CFCs)的变化。结果：TSPG 10、20、50和70μg/ml均可不同程度地提高细胞总数、CFCs和CD34^+细胞扩增倍数,TSPG 50μg/ml是最佳刺激浓度,可使细胞总数、CFCs和CD34^+细胞分别增至(2470．50±79．96)倍、(53．96±4．29)倍和(21．86士3．09)倍。结论：合适剂量的TSPG能够促进CD34^+造血干/祖细胞体外增殖。
Objective： To observe the effects of total saponins of panax ginseng （TSPG） in combination with hematopoietic growth factors （HGFs） on proliferation of CD34^＋ cells ex vivo. Methods： CD34^＋ cells were expanded by various concentrations of TSPG with combination of cytokines in liquid culture systems and the expanded cell number, CD34^＋ expansion times and the number of total CFC were determined. Results： TSPG （10, 20, 50, 70 μg/ml） raised the expanded cell number, CD34^＋ cell expansion times, and the numbers of total CFC. TSPG at 50μg/ml was identified as the most optimal concentration in those tested, and increased total nucleated cells by （ 2470. 50±79.96 ） times, CFC by （ 53. 96±4.29 ） times and CD34^＋ cells by （ 21.86±3. 09 ） times. Conclusion： This study demonstrates that TSPG can promote CD34^＋ cells proliferation by cooperating with hematopoietic growth factors.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2006年第4期430-432,449,共4页
Chinese Journal of Anatomy
基金
国家自然科学基金(38670880
30472253)
