期刊文献+

染料木黄酮对生长板软骨细胞胶原合成的影响及其机制 被引量:3

The effect and mechanism of genistein on collagen synthesis of growth plate chondrocytes
下载PDF
导出
摘要 目的探讨染料木黄酮(5,7,4′三羟基异黄酮)对大鼠肋生长板软骨细胞(ratcostochondralgrowthplatechondrocyte,RGC)胶原和Sox9表达的影响。方法原代培养RGC,3Hproline参入、RTPCR和Westernblot分别检测染料木黄酮对第1代RGC胶原合成、col2a1基因转录和Sox9表达的影响。结果染料木黄酮抑制RGC的胶原合成(P<0.05),col2a1的转录和Sox9的表达,其抑制作用随着浓度的升高而增强。结论染料木黄酮抑制培养RGC胶原合成的作用至少部分是通过下调Sox9的表达实现的。 Aim To investigate effects of genistein (5, 7, 4'-trihydroxyisoflavone) on rat costochondral growth plate chondrocyte (RGC) collagen and Sox9 expression. Methods Primary cultured RGC, effects of genistein on collagen synthesis, col2al mRNA transcription and Sox9 protein expression of 1st passage RGC were detected by isotope incorporation, RT-PCR and western blotting. Results Genistein inhibited collagen synthesis of 1st passage RGC (P 〈 0.05 ),col2al mRNA transcription and Sox9 protein expression. The inhibitory effect of genistein enhanced with its increasing concentration. Conclusion The inhibitory effect of genistein on collagen synthesis of RGC is partially mediated by the down-regulation of Sox9 expression.
出处 《中国药理学通报》 CAS CSCD 北大核心 2006年第8期927-929,共3页 Chinese Pharmacological Bulletin
基金 国家重点基础研究发展规划(973规划)资助项目(NoG1999054303) 广东省"十五"重大科技专项资助项目(NoA302020204) 暨南大学引进人才启动基金资助项目(No51205066)
关键词 染料木黄酮 生长板软骨细胞 Ⅱ型胶原 SOX9 genistein chondrocyte, growth plate collagen type Ⅱ Sox9
  • 相关文献

参考文献13

  • 1Smith E P,Boyd J,Frank G R,et al.Estrogen resistance caused by a mutation in the estrogen-receptor gene in a man[J].N Engl J Med,1994,331 (16):1056-61.
  • 2Sugimoto E,Yamaguchi M.Anabolic effect of genistein in osteoblastic MC3T3-E1 cells[J].Int J Mol Med,2000,5(5):515-20.
  • 3王增禄,孙纪元,王多宁,王剑波,谢艳华,王四旺,赵文明.槐角染料木素的结构鉴定及预防骨质疏松症的药理研究[J].中国药理学通报,2004,20(5):580-584. 被引量:12
  • 4季煜华,俞瑜,曾耀英.大鼠肋生长板软骨细胞的分离、培养及生物学特性的研究[J].中国病理生理杂志,2005,21(5):997-1000. 被引量:7
  • 5Nilsson A,Ohlsson C,Isaksson O G,et al.Hormonal regulation of longitudinal bone growth[J].Eur J Clin Nutr,1994,48(Suppl1):150-8.
  • 6Nilsson O,Abad V,Chrysis D,et al.Estrogen receptor-alpha and -beta are expressed throughout postnatal development in the rat and rabbit growth plate[J].J Endocrinol,2002,173 (3):407-14.
  • 7Morishima A,Grumbach M M,Simpson E R,et al.Aromatase deficiency in male and female siblings caused by a novel mutation and the physiological role of estrogens[J].J Clin Endocrinol Metab,1995,80 (12):3689-98.
  • 8Archer C W,Francis-West P.The chondrocyte[J].lnt J Biochem Cell Biol,2003,35(4):401-4.
  • 9Rickard D J,Monroe D G,Ruesink T J,et al.Phytoestrogen genistein acts as an estrogen agonist on human osteoblastic cells through estrogen receptors alpha and beta[J].J Cell Biochem,2003,89(3):633-46.
  • 10Nilsson O,Abad V,Chrysis D,et al.Estrogen receptor-alpha and -beta are expressed throughout postnatal development in the rat and rabbit growth plate[J].J Endocrinol,2002,173(3):407-14.

二级参考文献15

  • 1Siebler T, Robson H, Shalet SM, et al. Glucocorticoids, thyroid hormone and growth hormone interactions: implications for the growth plate[J]. Horm Res, 2001, 56 (Suppl 1): 7-12.
  • 2Garnero P, Rousseau JC, Delmas PD, et al. Molecular basis and clinical use of biochemical markers of bone, cartilage, and synovium in joint diseases[J]. Arthritis Rheum, 2000, 43(5): 953-968.
  • 3Archer CW, Francis-West P. The chondrocyte[J]. Int J Biochem Cell Biol, 2003, 35(4): 401-404.
  • 4de Haart M, Marijnissen WJ, van Osch GJ, et al. Optimization of chondrocyte expansion in culture. Effect of TGF beta-2, bFGF and L-ascorbic acid on bovine articular chondrocytes[J]. Acta Orthop Scand, 1999, 70(1): 55-61.
  • 5Saadeh PB, Brent B, Mehrara BJ, et al. Human cartilage engineering: chondrocyte extraction, proliferation, and characterization for construct development[J]. Ann Plast Surg, 1999, 42(5): 509-513.
  • 6Larson CM, Kelley SS, Blackwood AD, et al. Retention of the native chondrocyte pericellular matrix results in significantly improved matrix production[J]. Matrix Biol, 2002, 21(4): 349-359.
  • 7刘萍 边强 阮芝娟.国外药品最新开发动态[J].药学进展,2000,24(4):256-7.
  • 8刘中厚主编.骨质疏松学,北京:科学出版社,1998:454
  • 9徐叔云,卞如濂,陈修主编.药理实验方法,北京:人民卫生出版社,2002:1560-8
  • 10Ishimi Y,Arai N,Wang X,Wu J.Difference in effective dosage of genistein on bone and uterus in ovariectomized mice.Biochem Biophys Res Commun,2000:274(3):697~701

共引文献17

同被引文献30

  • 1季煜华,曾耀英.维生素C对大鼠肋骨生长板软骨细胞增殖和胶原合成的影响[J].基础医学与临床,2004,24(6):629-632. 被引量:11
  • 2Canalis E,Giustina A,Bilezikian J P.Mechanisms of anabolic therapies for osteoporosis[J].N Engl J Med,2007,357(9):905-16.
  • 3Krishnan V,Bryant H U,MacDougald O A.Regulation of bone mass by Wnt signaling[J].J Clin Invest,2006,16(5):1202-9.
  • 4Bennett C N,Longo K A,Wright W S,et al.Regulation of osteoblastogenesis and bone mass by Wnt10b[J].Proc Natl Acad Sci USA,2005,102(9):3324-9.
  • 5Canalis E,Economides A N,Gazzerro E.Bone morphogenetic proteins,their antagonists,and the skeleton[J].Endocr Rev,2003,24(2):218-35.
  • 6Kulkarni N H,Onyia J E,Zeng Q,et al.Orally bioavailable GSK-3alpha/beta dual inhibitor increases markers of cellular differentiation in vitro and bone mass in vivo[J].J Bone Miner Res,2006,21(6):910-20.
  • 7Rossouw J E,Anderson G L,Prentice R L,et al.Risks and benefits of estrogen plus progestin in healthy post-menopausal women:principal results from the women's health initiative randomized controlled trial[J].J Am Med Assoc,2002,288(3):321-33.
  • 8Wong R W,Rabie A B.Effect of genistein on bone formation[J].Front Biosci(Elite Ed),2010,2:764-70.
  • 9Yamaguchi A,Sakamoto K,Minamizato T,et al.Regulation of osteoblast differentiation mediated by BMP,Notch,and CCN3/NOV[J].J Dent Sci Rev,2008,44(1):48-56.
  • 10Tulac S,Nayak N R,Kao L C,et al.Identification,characterization,and regulation of the canonical Wnt signaling pathway in human endometrium[J].J Clin Endocrinol Metab,2003,88(8):3860-6.

引证文献3

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部