摘要
目的在人胚肾细胞系HEK-293中建立低密度脂蛋白受体基因表达的固醇调控报告系统,通过姜黄素的干预培养,从基因调控水平上阐明姜黄素降脂作用的机制。方法将绿色荧光蛋白报告基因构建在调控低密度脂蛋白受体基因表达的固醇调控元件的下游,经磷酸钙沉降法导入HEK-293细胞,在用含姜黄素的培养液中培养2 d后,用荧光显微镜及流式细胞仪对绿色荧光蛋白的表达情况进行定性定量分析。结果姜黄素诱导了绿色荧光蛋白的表达。结论姜黄素可以通过影响固醇调控元件的作用上调低密度脂蛋白受体基因的表达。
Aim To investigate the lipid-lowering mechanism of Chinese Medicine curcumin. A sterol regulatory report system of LDLR gene expression in HEK-293 cell line was established. Method A green fluorescent protein （GFP） gene was constructed downstream of the sterol regulatory element-1 （ SRE-1 ） and the constructed plasmid was introduced into HEK-293 cell line. This SRE-GFP transfected 293 cells were treated with different concentration of curcumin for 2 days. The expression of GFP in the cells was measured by a fluorescence microscope and Flow Cytometry. Results The expression of GFP in SRE-GFP transfected 293 cells was obviously induced by curcumin. Conclusion Curcumin may positively affect the expression of LDLR gene by its influence on SRE-1.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2006年第7期828-830,共3页
Chinese Pharmacological Bulletin
基金
浙江省自然科学基金资助项目(NoY204269)
浙江省中医药科研基金资助项目(No2005C004)
关键词
胚肾细胞
固醇调控元件
低密度脂蛋白受体
绿色荧光蛋白
姜黄素
HEK-293
sterol regulatory element-1
low density lipoprotein receptor
green fluorescence protein
curcumin
