摘要
研究了受蚕豆萎蔫病毒2号(Broadbeanwiltvirus2,BBWV2)中国分离物B935和欧洲分离物PV131侵染的蚕豆(Vicicafaba)叶片光合特性、叶绿素荧光诱导动力学参数和叶绿体超微结构变化。感病蚕豆叶绿素含量减少,叶绿素a/b比逐步降低;光合气体交换参数Pn值和Gs值降低,Ci值升高;叶绿素荧光诱导动力学参数Fv/Fm、Fv'/Fm'、ΦPSII、qP值均有不同程度降低,NPQ值升高;光合器结构遭到不同程度的破坏,B935侵染后叶绿体发育不良,片层结构疏松,PV131侵染后叶绿体肿胀变圆,片层结构疏松瓦解。与B935相比,PV131侵染对以上各参数的变化有更大影响,且对叶绿体的破坏更为严重。实验结果表明BBWV2不同分离物对光系统II(PSII)的抑制作用与光合器受损程度相关。
The effects of Broad bean wilt virus 2 （BBWV 2） isolate B935 and PV131 infection on photosynthetic activities and chloroplast ultrastructure in broad bean （Viciafaba） were investigated. As the disease progressed, net photosynthetic rate （Pn）, stomatal conductance （Gs） of leaves, the chlorophyll content and chlorophyll a/b ratio decreased while the intercellular CO2 concentration （Ci） of leaves rose in comparison with that of the healthy plants （Figs.l, 2）. After BBWV 2 infection, Fv/Fm, Fv＇/Fm＇, ΦPSII and qP values all became lower, but the NPQ values were higher, than the healthy control plants （Fig.3）. Electron microscopy showed that BBWV 2 infection disrupted the chloroplast structure. Most of the B935-infected chloroplasts showed inhibition of lamellar development or membrane vesiculation （Fig.4B, C） and PV131- infected chloroplasts showed swollen or disintegrated membrane （Fig.4D-F）. Chloroplasts of PV131-infected leaves were different from those of normal ones to a higher degree than those of the B935-infected ones, which suggests that the disruption of chloroplast structure by virus invasion is responsible for the suppression of photosynthesis, which is more serious with PV131 than with B935.
出处
《植物生理与分子生物学学报》
CAS
CSCD
北大核心
2006年第4期490-496,共7页
Journal Of Plant Physiology and Molecular Biology
基金
国家自然科学基金项目(Nos.30270873
30370305)资助。~~
关键词
蚕豆萎蔫病毒2号
蚕豆
叶绿体超微结构
光合作用
叶绿素荧光
Broad bean wilt virus 2
broad bean
chloroplast ultrastructure
photosynthesis
chlorophyll fluorescence
