摘要
目的：通过对犬胰腺微波固化后的机体生理状态及病理改变的观察,以期探讨微波固化应用于胰腺的可行性与安全性．方法：成年健康杂种犬15条,无菌手术暴露胰腺下叶后,利用微波探头以50W功率,1cm为间距固化5点,每点固化时间为3min．术后监测淀粉酶等血生化指标,并分别于术后即刻、1d及1wk各处死5条．利用HE法、NADH组织活性染色及TUNEL染色对固化中心区及固化旁组织进行病理分析．结果：术后即刻血淀粉酶开始升高,术后48-72h出现峰值,无并发症者均于1wk内降至正常,术后即刻可见固化区组织呈凝固性坏死,组织活性消失,与周围组织分解明显．1d后可于固化旁组织发现大量凋亡细胞．1wk后固化区纤维组织增生包裹．结论：微波固化治疗胰腺疾病是安全可行的．
AIM： To evaluate the feasibility and safety of microwave tissue coagulation to pancreas through the observation of physiological state and pathological changes after the coagulation of canine pancreas.
METHODS： Microwave tissue coagulation of fifteen healthy mongrel dogs＇ pancreas was performed hrough laparotomy. The power and time were set on 50 Watt and three minutes respectively. The 5 coagulated points were selected at an interval of 1cm, The blood amylase and other parameters were examined regularly after the operation. The dogs were killed at the instant time （n = 5）, the 1^st d （n = 5） and 1^st wk （n = 5） after the operation. Hematoxylin and eosin （HE） staining together with nicotinamide adenine dinucleotide （NADH） and TUNEL staining was used to observe the pathological changes.
RESULTS： The level of blood amylase increased immediately after the operation and the maximal value occurred within 48-72 h. Furthermore, it returned to normal level 1 wk later if no complications occurred. The segmental microwave lesions included hyperintense zone of coagulative necrosis and peripheral hypointense zone. Apoptosis was observed at the peripheral zone 1 d after the operation. The fibrosis and tissue absorption occurred finally 1 wk after the operation.
CONCLUSION： Microwave tissue coagulation is safe and feasible, and it may be used in the treatment of pancreatic cancer at the late stage.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第21期2073-2076,共4页
World Chinese Journal of Digestology
基金
哈尔滨市归国留学人员基金项目
No.2003AFLXJ007~~
关键词
微波固化
胰腺癌
犬胰腺
病理变化
Microwave tissue coagulation
Pancreatic cancer
Canine pancreas
Pathological changes
