普仑斯特对人支气管上皮细胞表达嗜酸性粒细胞趋化因子-3的影响被引量：2

Influence of Pranlukast on Expression of Eotaxin-3 in Bronchial Epithelia Cells

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摘要目的研究白三烯D4(LTD4)是否参与调节人支气管上皮细胞表达嗜酸性粒细胞趋化因子-3(Eot-3),探讨白三烯受体拮抗剂普仑斯特对此调节效应的影响。方法用LTD4预处理人支气管上皮细胞1h,用IL-4刺激细胞24h,酶联免疫吸附试验检测细胞培养上清中Eot-3蛋白水平;用不同水平普仑斯特预处理细胞,重复上述步骤。结果未经处理的人支气管上皮细胞仅表达微量Eot-3;用IL-4刺激细胞并培养24h后,Eot-3表达明显增强;LTD4预处理细胞1h,可使IL-4的诱导作用加强;普仑斯特可完全逆转LTD4的上调作用。结论上调IL-4诱导Eot-3在上皮细胞的表达可能是白三烯参与支气管哮喘嗜酸性粒细胞炎症的机制之一;普仑斯特对LTD4上调效应的逆转,可能是白三烯受体拮抗剂治疗哮喘的作用机制之一。 Objective To investigate whether leukotriene D4 （ LTTN ） regulates eotaxin - 3 （ Eot - 3 ） expression in bronchial epithelial cells, and study effect of pranlukst on the regulation. Methods BEAS 2B cells and normal human bronchial epithelia cells were pretreated with LTD4 for 1 hour,stimulated with interleukin 4, the cells were incubated for 24 hours. Eot - 3 protein in supernatant were measured by enzyme linked immunosorbent assay（ELISA）. The cells were pretreated with pranlukast in different concentration, then the above procedure was repeated. Results The untreated bronchial epithelial cell expressed Eot 3 protein on a very low level. After stimulating with IL 4 and incubating for 24 hours, Eot 3 production increased significantly. Pretreating the cells with LTD4 enhanced the inducing effect of IL- 4. Pranlukast inverted the upregulation of LTD4. Conclusions Upregulating the expression of Eot - 3 induced by IL - 4 on bronchial epithelial cells may explain partially the mechanism of leukotrienes involving airway allergic inflammation of asthma. The invertiun impact on upregulation of LTD4 by pranlukast may be one of mechanisms that leukotrienes receptor antagonist cure asthma.

作者欧维琳韦欢冯智英朱春江郑明慈

机构地区桂林医学院附属医院儿科

出处《实用儿科临床杂志》 CAS CSCD 北大核心 2006年第16期1070-1071,共2页 Journal of Applied Clinical Pediatrics

关键词支气管上皮细胞白三烯D4 白细胞介素-4 嗜酸性粒细胞趋化因子-3 普仑斯特 bronchial epithelial cell leukotrienes D4 interleukin - 4 eotaxin - 3 pranlukast

分类号 R96 [医药卫生—药理学]

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参考文献13

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