摘要
目的探讨自体激活雪旺细胞(autologousactivatedSchwanncells,AASCs)移植治疗急性脊髓损伤的疗效。方法通过结扎单侧隐神经从而激活自体雪旺细胞并进行体外分离、培养及纯化,测定其不同培养时期培养基中神经生长因子、脑源性神经营养因子含量的变化。Wistar大鼠90只,以纽约大学脊髓损伤打击器建立T10急性脊髓损伤模型。随机分为3组,每组30只:单纯DMEM移植对照组、自体未激活雪旺细胞(autologousSchwanncells,ASCs)移植组和AASCs移植组。对各组实验动物脊髓损伤后肢体功能的恢复情况进行行为学评分(BBB评分)、体感诱发电位与运动诱发电位(somatosensoryevokedpotentialandmotorevokedpotentials,SEP&MEP)、生物素标记的葡聚糖胺示踪皮质脊髓束(cor-ticospinaltract,CST)观察,比较各组差异。结果BBB评分4周以后组间比较差异有统计学意义(P<0.05),其中细胞移植组明显高于对照组(P<0.05);SEP、MEP潜伏期和波幅值8、12周后组间比较差异有统计学意义(P<0.05);CST标记距离损伤中心向头端方向0.6、1.2、1.8、2.4mm和距离损伤中心向尾端方向0.6、1.2、1.8mm组间比较差异有统计学意义(P<0.05)。结论AASCs可分泌大量神经营养因子,并能明显促进急性脊髓损伤后的轴突再生和肢体功能恢复。
Objective To observe the effect of treating acute spinal cord injury in rats by transplantation of autologous activated Schwann cells（AASCs）. Methods Unilateral saphenous nerves were ligated directly, free it and culture Schwann cells 1 week later using the tissue clot method. Nerve growth factor（NGF） and brain-derived neurotrophic factor（BDNF） in medium were detected in different periods. 90 female Wistar rats[（200±30） g] were randomly assigned to 3 different study groups as follows： control group A（n=30）： 20% DMEM injection; research group B（n=30）： autologous Schwann cells（ASCs） transplantation; research group C （n=30）： AASCs transplantation. The cells were purified before transplantion to the injuried T10 spinal cord site of rats （New York University type weight drop apparatus, NYU）. The recoveries of the lower extremity were observed using Basso-Beattie-Bresnahan（BBB） locomotor scoring system and somatosensory evoked potential and motor evoked potentials（SEP ＆ MEP）. And then observe the coticospinal tract（CST） using the biotinylated dextran amine （BDA） tracing. Results BBB score was higher in research group than the control group 4 weeks after injury, the statistical difference was significant （P〈 0.05）. Statistical differences showed in latency period and wave amplitude in SEP ＆ MEP after 8 and 12 weeks. Using tracing 7 points in different distance spreading from the lesion center in each group to measure the CST, the results showed more axons regenerate in AASCs group than the other two groups with significant statistical difference （P〈 0.05）. Conclusion ASCs and AASCs can survive and keep active after transplantation; they both can promote the recovery of the paralyzed lower extremity after spinal cord injury, and the effect of AASCs is better.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
2006年第8期553-557,共5页
Chinese Journal of Orthopaedics
基金
国家自然科学基金资助项目(30271313)
吴阶平临床医学基金资助项目(TJB-2004-1)
天津市科委基金资助项目(023802811)
关键词
许旺细胞
细胞移植
脊髓损伤
轴突
Schwann cells
Cell transplantation
Spinal cord injuries
Axons
