摘要
目的:通过研究精子正常和异常男性精浆和精子中尿激酶及受体含量差异,以了解尿激酶及受体与男性生育力的关系。方法:采用双抗体夹心ELISA法测定22例正常生育男性和44例少弱精子症男性精浆和精子中尿激酶及受体的含量。结果:①正常男性精浆尿激酶平均含量为(4 803.69±602.78)mU/L,与少弱精子症组[(4 061.35±736.23)mU/L]相比,差异有显著性(P<0.01)。正常生育男性精子尿激酶平均含量为(30.29±3.16)mU/106个精子,与少弱精子症组[(20.51±4.2)mU/106个精子],差异有显著性(P<0.01)。②正常生育男性精子尿激酶受体平均含量为(12.97±3.11)mU/106个精子相比,与少弱精子症组[(6.09±1.45)mU/106个精子]相比,差异有显著性(P<0.01)。③精子和精浆中尿激酶含量和精子活率和活力呈显著正相关。结论:尿激酶和男性生育力相关,少弱精子症和正常生育男性精液中尿激酶及其受体含量存在差异。
Objective ： To find the difference between the levels of urokinase-type plasminogen activator （uPA） and urokinase-type plasminogen activator receptor（uPAR） in the seminal plasma and sperm of fertile and oligoasthenozoospermia men, and to understand their correlation with male fertility. Methods ： The levels of uPA in the seminal plasma and sperm of 22 normospermic males and 44 oligoasthenozoospermia patients were measured by ELISA. Results ： ①The average level of uPA in the seminal plasma and sperm of the normospermic group, （ [4 803.69 ± 602.78 ] mU/L） and （ [ 30.29 ± 3.16 ] mU/10^6 sperm） were higher than those of the oligoasthenozoospermia group, （ [ 4 061.35 ± 736.23 ] roD/L）, and （ [ 20.51 ± 4.2 ] mU/10^6 sperm ） （ P 〈 0.01 ）. ②The average level of uPAR in the sperm of the normospermic group（ [ 12.97 ± 3.11 ] mU/10^6 sperm） was significantly higher than that of the oligoasthenozoospermia group, （ [6.09 ± 1.45 ] mU/10^6 sperm） （P 〈0.01 ）. ③The levels of uPA and uPAR in the sperm and the content of uPA in the seminal plasma were positively correlated with sperm motility and viability. Conclusion： Urokinase-type plasminogen activator is related with male fertility and the levels of uPA and uPAR vary in the seminal plasma and sperm of fertile and oligoasthenozoospermia males.
出处
《中华男科学杂志》
CAS
CSCD
2006年第9期791-793,共3页
National Journal of Andrology
基金
国家"十五"攻关计划课题(2004BA720A33-1)
关键词
尿激酶
尿激酶受体
少弱精子症
精浆
精子
urokinase-type plasminogen activator
urokinase-type plasminogen activator receptor
oligoasthenozoospermia
seminalplasma
sperm
