摘要
目的:探讨罗丹明/碘化吡啶(Rh123/PI)双染法检测精子线粒体膜功能的可行性及临床意义。方法:收集63例精液标本,按WHO精液分析标准分成正常组(n=31)与异常组(n=32)。精子用Rh123/PI染色,流式细胞仪(FCM)分析。结果:正常组与异常组精子Rh123+PI-、Rh123-/PI+、Rh123-/PI-百分率均存在统计学差异(P均<0.05);Rh123+PI-与精子活动率呈正相关(r=0.549,P=0.000),与(a+b)级精子百分率呈正相关(r=0.531,P=0.000),与d级精子百分率呈负相关(r=-0.586,P=0.000);Rh123-/PI+与精子活率负相关(r=-0.586,P=0.000),与(a+b)级精子百分率负相关(r=-0.594,P=0.000),与d级百分率精子正相关(r=0.585,P=0.000),Rh123-/PI-仅与异常组的密度有相关性(r=-0.563,P=0.001)。结论:运用Rh123/PI双染法鉴定精子的线粒体膜功能具有可行性,可用于评估男性生育力。
Objective： To investigate the feasibility and clinical significance of detecting sperm mitochondrial function by using Rh123/PI dual fluorescent staining and flow cytometry analysis, and to explore the relationship between the results of Rh123/PI dual fluorescent staining and seminal parameters. Methods： Sixty-three semen samples were classified as normal （n = 31 ） and abnormal （n =32） according to the World Health Organization guidelines. Rh123/PI dual fluorescent staining was then carried out to evaluate sperm mitochondrial function by flow cytometry analysis. Results： Significant differences in Rh123 ^＋ PI^- , Rh123-/PI ^＋ and Rh123-/PI^- were detected between the normal and abnormal semen samples（ P 〈 0.05 ）. There was a significant positive correlation between the Rh123 ^＋ PI^- sperm and sperm motility and a significant inverse correlation between Rh123 ^＋ PI^- and immotile sperm. But the Rh123 - PI ^＋ sperm showed a contrary relationship with Rh123 ^＋ PI^-. A significant inverse correlation was also observed between the Rh123-/PI^- sperm and sperm concentration in the abnormal group. Conclusion： Rh123/PI dual fluorescent staining and flow cytometry analysis can readily and quickly detect sperm mitochondrial function and be used to evaluate semen quality.
出处
《中华男科学杂志》
CAS
CSCD
2006年第9期803-806,共4页
National Journal of Andrology
基金
江苏省"六大人才高峰"资助项目(2005A5)
