摘要
目的对可手术的乳腺癌患者进行骨髓播散肿瘤细胞的检测,并对骨髓免疫微环境进行研究。方法联合应用上皮膜抗原(EMA)和细胞角蛋白19(CK19),采用免疫组织化学的方法检测骨髓中的微小转移灶,用流式细胞仪的方法比较不同组间免疫指标的差异。结果72例乳腺癌患者中,30例(41．7％)骨髓中存在微小转移灶。原发灶肿块大于5 cm,p53蛋白表达阴性的患者易发生骨髓微小转移。在乳腺癌患者中,骨髓播散肿瘤细胞阳性的患者比阴性的患者具有较高的记忆CD4^+T细胞比例,记忆CD4^+T细胞/记忆CD8^+T细胞比值。结论联合应用EMA和CK19进行骨髓播散肿瘤细胞检测的方法是可行的。骨髓播散肿瘤细胞转变成为大体转移的患者,是否与其记忆T细胞水平低下相关需作进一步研究。
Objective To detect minimal residual disease and immunologic microenvironmental characteristics in bone marrow of breast cancer. Methods Disseminated tumor cells were detected by using immunoeytoehemical staining of EMA and CK19, and the immunological changes were analyzed by using flow cytometry. Results By using the immunoeytoehemical staining of EMA and CK19, micrometastases were detected in 30 out of 72 （41.67 % ） breast cancer patients. It was found primary tumor size and P53 protein expression were significantly associated with the incidence of micrometastases. The percentage of memory CD4^＋T cells and the ratio of the memory helper T cells and memory suppressor T cells were significantly higher in micrometastases positive group than that in micrometastases negative group. Conclusion Our method is proved to be acceptable. Whether different levels of memory cell will affect the outcome of disseminated tumor cells is still under investigation.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第9期1062-1063,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金(30025015)
上海市科委资助项目(014119032)
关键词
乳腺癌
骨髓
转移
免疫学
Breast carcinoma
Bone marrow
Metastase
Immunology
